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Inhibitory effect of 1α,25-dihydroxyvitamin D3 on mast cell proliferation and A23187-induced histamine release, also accompanied by a decreased c-kit receptor

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Abstract

Using mouse peritoneal mast cells, we investigated the effects of 1α,25-dihydroxyvitamin D3 (calcitriol) on mast cell proliferation and histamine release. Calcitriol did not affect IL-3/IL-4-dependent mast cell proliferation, but it selectively inhibited stem cell factor-dependent mast cell proliferation and colony formation. Immunohistochemical and immunoblot analyses revealed that calcitriol treatment reduced expression of purified peritoneal mast cell c-kit protein. Using a mast cell line, MC/9, both c-kit protein and c-kit mRNA transcript were seen to be reduced following calcitriol treatment. Calcitriol also reduced histamine release induced by calcium ionophore A23187. In contrast, anti-IgE antibody-dependent histamine release was not affected by calcitriol. Our results indicate that calcitriol inhibits mast cell proliferation and A23187-induced histamine release that might be associated with a decreased expression of c-kit receptor.

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Authors and Affiliations

  1. Department of Dermatology, Asahikawa Medical College, 4-5-3-11, Nishikagura, Asahikawa, Hokkaido, Japan

    Noriaki Toyota, Hiroyuki Sakai, Hidetoshi Takahashi, Yoshio Hashimoto & Hajime Iizuka

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  1. Noriaki Toyota
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  4. Yoshio Hashimoto
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Toyota, N., Sakai, H., Takahashi, H. et al. Inhibitory effect of 1α,25-dihydroxyvitamin D3 on mast cell proliferation and A23187-induced histamine release, also accompanied by a decreased c-kit receptor. Arch Dermatol Res 288, 709–715 (1996). https://doi.org/10.1007/BF02505282

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  • DOI: https://doi.org/10.1007/BF02505282

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