Changes in keratinocyte differentiation following mild irritation by sodium dodecyl sulphate
- 117 Downloads
Although the induction of acute irritant dermatitis by detergents has been studied extensively in recent years, our understanding of the cell biological events in the repair phase, and its relevance for the development of chronic irritant dermatitis is limited. Here we studied the reaction pattern of human skin to short-term application of sodium dodecyl sulphate (SDS) in a model that induced a minimal acute inflammatory reaction (absence of polymorphonuclear leucocytes, PMN) and did not have cytopathic effects on the epidermal keratinocytes as determined by histological investigation. All parameters were measured up to 14 days after exposure to SDS. Application of SDS caused disturbances of barrier function as measured by transepidermal water loss and had vascular effects as judged by erythema. Several cell biological markers for epidermal growth and differentiation were examined by immunohistochemistry. A rapid and strong induction of the cornified envelope precursor protein involucrin was seen in the stratum spinosum, with a peak at 24 h. Within 24 h a strong upregulation of epidermal fatty acid binding protein (E-FABP) was noted, with a peak at 7 days after injury. Cellular proliferation in the basal layer was increased fivefold as assessed by nuclear staining for the Ki-67 antigen, showing a peak at 48 h. Surprisingly, no significant induction of cytokeratin 16 and SKALP/elafin expression, two markers associated with epidermal hyperproliferation and inflammation, was seen. These findings suggest that the cellular changes following exposure to detergent are distinct from those seen in other forms of skin injury. We would speculate that the epidermal response to detergent exposure is primarily directed at restoration of barrier function.
Key wordsIrritant dermatitis Keratinocytes Sodium Dodecyl sulphate
- 3.Bergen LH van, Andriessen MA, Bergers M, Spruijt KIJ, Latijnhouwers MAHE, Kerkhof PCM van de, Schalkwijk J (1994) (abstract) Expression of skin-derived antileucoproteinase/elafin during wound healing in human skin. J Invest Dermatol 103:431Google Scholar
- 12.Madsen P, Rasmussen HH, Leffers H, Honore B, Celis JE (1992) Molecular cloning and expression of a novel keratinocyte protein (psoriasis-associated fatty acid-binding protein [PA-FABP]) that is highly up-regulated in psoriatic skin and that shares similarity to fatty acid-binding proteins. J Invest Dermatol 99:299–305PubMedCrossRefGoogle Scholar
- 26.Van Duijnhoven Avontuur WM, Alkemade JA, Schalkwijk J, Mier PD, Valk PG van der (1994) The inflammatory and proliferative response of normal skin in a model for acute chemical injury: ornithine decarboxylase induction as a common feature in various models for acute skin injury. Br J Dermatol 130:725–730PubMedCrossRefGoogle Scholar