Abstract
Using immunoblot and immunofluorescence analysis, we demonstrated the presence and localization of an immunoreactive form of erythrocyte ankyrin in human epidermal keratinocytes. Immunoblot analysis revealed that both human epidermis and cultured epidermal keratinocytes contained ankyrin-like proteins of molecular mass 210 kDa that crossreacted with antihuman erythrocyte ankyrin antibodies. Immunofluorescence microscopy revealed that the plasma membrane of epidermal keratinocytes was stained. Eccrine sweat gland cells and ductal cells were also stained. These results indicate that in human epidermal keratinocytes, eccrine sweat gland cells and ductal cells, an ankyrin-like protein is present as one of the membrane proteins. The present findings and our recent previous studies showing the presence of a spectrin-like protein (fodrin) and 4.1-like proteins in these cells enable us to suggest that a membrane skeletal protein lattice may exist in these cells.
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Alper SL, Stuart-Tilley A, Simmons CF, Brown D, Drenckhahn D (1994) The fodrin-ankyrin cytoskeleton of choroid plexus preferentially colocalizes with apical Na+K+-ATPase rather than with basolateral anion exchanger AE2. J Clin Invest 93: 1430–1438
Bennett V (1985) The membrane skeleton of human erythrocytes and its implications for more complex cells. Annu Rev Biochem 54: 273–304
Bennett V (1989) The spectrin-actin junction of erythrocyte membrane skeletons. Biochim Biophys Acta 988: 107–121
Bennett V (1992) Ankyrins: adaptors between diverse plasma membrane proteins and the cytoplasm. J Biol Chem 267: 8703–8706
Bennett V, Davis J (1981) Immunoreactive forms of human erythrocyte ankyrin are localized in mitotic structures in cultured cells and are associated with microtubules in brain. Cold Spring Harb Symp Quant Biol 46: 647–657
Bennett V, Davis J (1981) Erythrocyte ankyrin: immunoreactive analogues are associated with mitotic structures in cultured cells and with microtubules in brain. Proc Natl Acad Sci USA 78: 7550–7554
Bourguignon LYW, Walker G, Suchard S, Balazovich K (1986) A lymphoma plasma membrane-associated protein with ankyrin-like properties. J Cell Biol 102: 2115–2124
Davis J, Davis L, Bennett V (1989) Diversity in membrane binding sites of ankyrins. J Biol Chem 264:6417–6426
Drenckhahn D, Bennett V (1987) Polarized distribution of Mr 210000 and 190000 analogs of erythrocyte ankyrin along the plasma membrane of transporting epithelia, neurons and photoreceptors. Eur J Cell Biol 43: 479–486
Green KJ, Parry DAD, Steinert PM, Virata MLA, Wagner RM, Angst BD, Milles LA (1990) Structure of the human desmoplakins: implications for function in the desmosomal plaque. J Biol Chem 265: 2603–2612
Kaiser NW, O'Keefe E, Bennett V (1989) Adducin: Ca2+-dependent association with sites of cell-cell contact. J Cell Biol 109: 557–569
Kalomiris EL, Bourguignon LYW (1988) Mouse T-lymphoma cells contain a transmembrane glycoprotein (GP85) that binds ankyrin. J Cell Biol 106: 319–327
Ketis NV, Hoover RL, Karnovsky MJ (1986) Isolation of bovine aortic endothelial cell plasma membranes: identification of membrane-associated cytoskeletal proteins J Cell Physiol 128: 162–170
Koob R, Zimmermann M, Schoner W, Drenckhahn D (1987) Colocalization and coprecipitation of ankyrin and Na+, K+-ATPase in kidney epithelial cells. Eur J Cell Biol 45: 230–237
Koob R, Kraemer D, Trippe G, Aebi U, Drenckhahn D (1990) Association of kidney and parotid Na+, K+-ATPase microsomes with actin and analogs of spectrin and ankyrin. Eur J Cell Biol 53: 93–100
Kordeli E, Davis J, Trapp B, Bennett V (1990) An isoform of ankyrin is localized at nodes of ranvier in myelinated axons of central and peripheral nerves. J Cell Biol 110: 1341–1352
Lux SE, John KM, Bennett V (1990) Analysis of cDNA for human erythrocyte ankyrin indicates a repeated structure with homology to tissue-differentiation and cell-cycle control proteins. Nature 344: 36–42
Morrow JS, Cianci CD, Ardito T, Mann AS (1989) Ankyrin links fodrin to the alpha subunit of Na, K-ATPase in Madin-Darby canine kidney cells and intact renal tubule cells. J Cell Biol 108: 455–465
Nelson WJ, Hammerton RW (1989) A membrane-cytoskeletal complex containing Na, K-ATPase, ankyrin, and fodrin in Madin-Darby canine kidney (MDCK) cells: implications for the biogenesis of epithelial cell polarity. J Cell Biol 108: 893–902
Nelson WJ, Lazarides E (1984) Goblin (ankyrin) in striated muscle: identification of the potential membrane receptor for erythroid spectrin in muscle cells. Proc Natl Acad Sci USA 81: 3292–3296
Nelson WJ, Veshnock PJ (1987) Ankyrin binding to (Na++K+)-ATPase and implications for the organization of membrane domains in polarized cell. Nature 328: 533–536
Shimizu T, Takakuwa Y, Koizumi H, Ishibashi T, Ohkawara A (1990) Immunohistochemical analysis of human skin using antispectrin and anti-β-fodrin antibodies. Arch Dermatol Res 282: 274–277
Shimizu T, Takakuwa Y, Koizumi H, Ishibashi T, Ohkawara A (1991) Presence and localization of proteins immunologically related to erythrocyte protein 4.1 in human skin. Histochemistry 95: 549–554
Srinivasan Y, Elmer L, Davis J, Bennett V, Angelids K (1988) Ankyrin and spectrin associate with voltage-dependent sodium channels in brain. Nature 333: 177–180
Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350–4354
Tyler JM, Hargreaves WR, Branton D (1979) Purification of two spectrin-binding proteins: biochemical and electron microscopic evidence for site-specific reassociation between spectrin and band 2.1 and 4.1 Proc Natl Acad Sci USA 76: 5192–5196
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Shimizu, T., Takakuwa, Y., Koizumi, H. et al. Immunoreactive analogues of erythrocyte ankyrin in human epidermal keratinocytes. Arch Dermatol Res 288, 19–23 (1996). https://doi.org/10.1007/BF02505038
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DOI: https://doi.org/10.1007/BF02505038