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Chromosome banding in the genusPinus

I. Identification of chromosomes inP. nigra by fluorescent banding method

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Abstract

Somatic chromosomes ofPinus nigra var.maritima (2n=24) were sequentially stained with DNA binding base-specific fluorochromes, chromomycin A3 (CMA) and DAPI. Many CMA- and DAPI-bands appeared at intercalary and/or proximal regions of most chromosomes. These banding patterns reversely related. Individual chromosomes were easily identified using these fluorescent banding patterns.

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Abbreviations

AT:

adenine-thymine

CMA:

chromomycin A3

DAPI:

4′,6-diamidino-2-phenylindole

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Authors and Affiliations

  1. Biological Institute, Faculty of Education, Ehime University, 790, Matsuyama

    Masahiro Hizume, Atsuo Ohgiku & Akio Tanaka

Authors
  1. Masahiro Hizume
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  2. Atsuo Ohgiku
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  3. Akio Tanaka
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Hizume, M., Ohgiku, A. & Tanaka, A. Chromosome banding in the genusPinus . Bot Mag Tokyo 96, 273–276 (1983). https://doi.org/10.1007/BF02499006

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  • DOI: https://doi.org/10.1007/BF02499006

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