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Geschlechtsunterschiede Bei Der Neuromodulation von Mukosamastzellen im Rattenjejunum

Different response of mucosal mast cells in rat jejunum to electrical stimulation of the cervical vagal nerves depending on sex

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Abstract

The effect of electrical stimulation of both cervical vagal nerves on mucosal mast cells in the jejunum was investigated in an in vivo animal model with rats of both sexes. Males showed a significant increase of mast cell densities after electrical stimulation (1.0 mA, 5 Hz, 5 ms, 12 min) in the lamina propria. Simultaneously, we observed a significant increase of tissue histamine levels (ANOVA:P<0.05), whereas serum levels remained unchanged. However, even though females had significantly higher levels throughout compared to males (ANOVA;P<0.05), they did not show any significant reaction to electrical stimulation. These in vivo data support morphological and in vitro data from other investigators, who hypothesized a functional interaction between mucosal mast cells and nerves. However, degranulation seems to be a poor in situ indicator for mast-cell stimulation, as mastcell densities increased in males, while the percentage of degranulated cells remained the same in all groups (about 40%). Instead, electrical stimulation of the vagal nerve seems to trigger histamine synthesis, or simply stabilization of mast cells. Interestingly, this phenomenon seems to be sex-dependent, suggesting a regulatory role for sex hormones in this scenario.

Zusammenfassung

Im Rahmen einer tierexperimentellen Studie an der Ratte wurde der Effekt einer elektrischen In-vivo-Stimulation des zervikalen N. vagus auf die Mukosamastzellen im Jejunum untersucht. Dabei zeigten männliche Tiere nach Stimulation mit 1,0 mA (5 Hz, 5 ms, 12 min) eine deutliche Zunahme der Mastzelldichte in der Lamina propria; gleichzeitig kam es zu einem signifikanten Anstieg des Gewebehistaminspiegels (ANOVA:p<0,05), während die Serumspiegel nicht signifikant anstiegen. Anders sah dies bei den weiblichen Tieren aus, die zwar im Vergleich zu den Männchen durchweg höhere Ausganswerte boten (p<0,05), bei denen jedoch die elektrische Stimulation keine signifikanten Veränderungen bewirkte. Diese Studie bestätigt in vivo die Bedeutung morphologischer und funktioneller In-vitro Daten, die eine funktionelle Interaktion zwischen Mukosamastzellen und Nerven nahelegen. Im Gegensatz zu den klassischen allergischen Reaktionen löst die elektrische Stimulation jedoch offensichtlich keine Degranulation der Mastzelle aus (ca. 40% degranulierte Mastzellen in allen Gruppen), sondern eher eine Stabilisierung oder verstärkte Histaminsynthese der Mastzellen. Zusätzlich scheinen die Phänomene der Neuroimmunmodulation teilweise von Sexualhormonen beeinflußt zu werden, wie die Geschlechtsunterschiede nahelegen.

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Authors and Affiliations

  1. Chirurgische Klinik, Abteilung Allgemeinchirurgie, Eberhard-Karls-Universität, Hoppe-Seyler-Straße 3, D-72076, Tübingen

    T. Gottwald, H. -D. Becker & R. H. Stead

  2. Department of Pathology, McMaster University, Holburn Biomedical Corporation, Bownville, Ontario, Canada

    R. H. Stead & R. H. Stead

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  1. T. Gottwald
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  2. H. -D. Becker
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  3. R. H. Stead
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  4. R. H. Stead
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Gottwald, T., Becker, H.D., Stead, R.H. et al. Geschlechtsunterschiede Bei Der Neuromodulation von Mukosamastzellen im Rattenjejunum. Langenbecks Arch Chiv 382, 157–163 (1997). https://doi.org/10.1007/BF02498669

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  • DOI: https://doi.org/10.1007/BF02498669

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