Abstract
Phytotoxicity of metolachlor [2-chloro-N-(2-ethyl-6-methylphenyl)-N-(2-methoxy-1-methylethyl)acetamide] toward grain sorghum [Sorghum bicolor (L.) Moench] increased as soil moisture increased. This was found with both unprotected sorghum and sorghum protected with 1.25 g CGA-43089 [α([cyanomethoxy]imino)benzeneacetonitrile] per kg of seed. However, under all conditions, metolachlor was less phytotoxic to protected sorghum than to unprotected sorghum. Metolachlor in sorghum coleoptiles increased as soil water increased. The rate of absorption of metolachlor and the total amount accumulated by excised sorghum coleoptiles was decreased by CGA-43089. Initial uptake of leucine by excised sorghum coleoptiles was decreased by metolachlor or metolachlor plus CGA-43089 but, after 24 h, uptake of leucine was increased by these treatments. Leucine incorporation into protein by coleoptiles was increased after 24 h treatment with CGA-43089. The apparent competitive effect of CGA-43089 on the absorption of metolachlor was most evident in the roots. Leakage of photosynthate from roots was highest following treatment with both CGA-43089 and metolachlor. Metolachlor did not increase leakage of labeled carbon from roots as compared with the control. These data indicate that the decreased rate of uptake of metolachlor in the presence of CGA-43089 was not a direct effect on cell permeability.
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References
Abdul-Baki AA (1979) An effective method for sterilizing seeds prior to use. Agron Abstr: 113
Armstrong TF, Meggitt WF, Penner D (1973) Absorption, translocation, and metabolism of alachlor by yellow nutsedge. Weed Sci 21:357–360
Balke NE (1980) Mechanism of inhibition of ion absorption by alachlor. Abstr Weed Sci Soc Am p. 84.
Chandler JM, Basler E, Santelmann PW (1974) Uptake and translocation of alachlor in soybeans and wheat. Weed Sci 22:253–258
Dick PS, apRees T (1975) The pathway of sugar transport in roots ofPisum sativum. J Exp Bot 26:305–314
Ebert E (1980) Herbicidal effects of metolachlor (2-chloro-N-(2-ethyl-6-methylphenyl)-N-(2-methoxy-1-methylethyl)acetamide) at the cellular level in sorghum. Pestic Biochem Physiol 13:227–231
Ellis JF, Peek JW, Boehle J, Muller G (1980) Effectiveness of a new safener for protecting sorghum from metolachlor injury. Weed Sci 28:1–5
Goeschl JD, Magnuson CE, DeMichele DW, Sharpe PJH (1976) Concentration-dependent unloading as a necessary assumption for a closed form mathematical model of osmotically driven pressure flow in phloem. Plant Physiol 58:556–562
Gruenhagen RD, Moreland DE (1977) Protein and nucleic acid synthesis in excised plant tissue. In: Truelove (ed) Research methods in weed science, ed 2. South Weed Sci Soc, Auburn, Alabama, pp 153–154
Hamill AS, Penner D (1973) Interaction of alachlor and carbofuran. Weed Sci 21:330–335
Hamm PC, (1974) Discovery, development, and current status of the chloroacetamide herbicides. Weed Sci 22:541–545
Hoagland DR, Arnon DI (1950) The water culture method of growing plants without soil. Univ Calif Agric Exp Stn Circ 347, 32 pp
Jaworski EG (1969) Analysis of the mode of action of herbicidal α-chloroacetamides. J Agric Food Chem 17:165–170
Jaworski EG (1975) Chloroacetamides. In Kearney PC, Kaufman DD (eds) Herbicides: Chemistry, degradation and mode of action, ed 2. Marcel Dekker, Inc, New York, vol 1, pp 349–376
Keener ME, DeMichele DW, Sharpe PJH (1979) Sink metabolism: A conceptual framework for analysis. Ann Bot 44:659–669
Ketchersid ML, Norton K, Merkle MG (1981) Influence of soil moisture on the safening effect of CGA-43089 in grain sorghum. Weed Sci 29:281–286
Lowry OH, Rosebrough NJ, Farr AL, Randall RL (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Morrod RS (1976) Effects of plant cell membrane structure and function. In Aurus LJ (ed) Herbicides: Physiology, biochemistry, ecology, ed 2. Academic Press, New York, vol 1, pp 281–304.
Pillai CGP, Davis DE (1975) Mode of action of CGA-18762, CGA-17020, and CGA-24705. Proc South Weed Sci Soc 28:308–314
Pillai CGP, Davis DE, Truelove B (1977) Site of uptake and mode of action of metolachlor. Proc South Weed Sci Soc 30:367
Pillai P, Davis DE, Truelove B (1979) Effects of metolachlor on germination, growth, leucine uptake, and protein synthesis. Weed Sci 27:634–637
Truelove B, Diner AM, Davis DE, Weete JD (1979) Metolachlor, membranes, and permeability. Abstr Weed Sci Soc Am p 99
Winkle ME, Leavitt JRC, Burnside OC (1980) Acetanilide-antidote combinations for weed control in corn (Zea mays) and sorghum (Sorghum bicolor) Weed Sci 28:699–704
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Paper No. TA17232, Texas Agric Exp Stn, Texas A & M University. This paper is taken in part from the PhD dissertation of M. L. Ketchersid.
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Ketchersid, M.L., Vietor, D.M. & Merkle, M.G. CGA-43089 effects on metolachlor uptake and membrane permeability in grain sorghum (Sorghum bicolor). J Plant Growth Regul 1, 285–294 (1982). https://doi.org/10.1007/BF02498657
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DOI: https://doi.org/10.1007/BF02498657