Chromatographia

, Volume 56, Issue 9–10, pp 591–595 | Cite as

Screening and mass-spectral confirmation of betalains in cactus pears

  • J. A. Fernández-López
  • R. Castellar
  • J. M. Obón
  • L. Almela
Originals Column Liquid Chromatography

Summary

The presence of betalain pigments in fruits ofOpuntia stricta, Opuntia undulata andOpuntia ficus-indica has been studied. Cultivars of deep red-purple colour, with high betacyanin content, were choosen for this investigation. The total betacyanin content was estimated spectrophotometrically E 1cm 1% =1120) and the results obtained indicated a higher betacyanin content inO. stricta (70 mg · 100 g−1 fresh fruit), than inO. undulata andO. ficus-indica (14–19 mg · 100 g−1 fresh fruit). Individual pigment analysis by HPLC with photodiode array detection revealed substantial differences between these species. In cactus pears fromO. stricta only betanin and isobetanin were detected. However, in cactus pears fromO. undulata andO. ficus-indica the predominant pigments were betanin and indicaxanthin, with very low levels of isobetanin. The identity of all these pigments was confirmed by mass-spectral analysis. The presence of neobetanin was not detected in fruits of any of the three species considered.

Key Words

Column liquid chromatography Electrospray mass spectroscopy Betalains Cactaceae Opuntia 

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References

  1. [1]
    Piatelli, M. InChemistry and Biochemistry of Plant Pigments: Goodwin, T.W. Eds, Academic Press, London,1976.Google Scholar
  2. [2]
    Strack, D.; Steglich, W.; Wray, V. InMethods in Plant Biochemistry (vol. 8): Dey, P.M.; Harborne, J.B. Eds, Academic Press, London,1993.Google Scholar
  3. [3]
    Von Elbe, J.H.; Klement, J.T.; Amundson, C.H.; Cassens, R.G.; Lindsay, R.C.J. Food Sci. 1974,39, 128–132.CrossRefGoogle Scholar
  4. [4]
    Von Elbe, J.H.Food Technol. 1975,29, 42–46.Google Scholar
  5. [5]
    Wissgott, U.; Bortlik, K.Trends Food Sci. Technol. 1996,7, 298–302.CrossRefGoogle Scholar
  6. [6]
    Jackman, R.L.; Smith, J.L. InNatural Food Colorants, 2nd Ed.: Hendry, G.A.F.; Houghton, J.D. Eds., Blackie, London,1996.Google Scholar
  7. [7]
    Odoux, E.; Domínguez-López, A.Fruits 1996,51, 61–78.Google Scholar
  8. [8]
    Reynoso, R.; García, F.A.; Morales, D.; González de Mejía, E.J. Agric. Food Chem. 1997,45, 2884–2889.CrossRefGoogle Scholar
  9. [9]
    Fernández-López, J.A.; Almela, L.J. Chromatogr. A 2001,913, 415–420.CrossRefGoogle Scholar
  10. [10]
    Escribano, J.; Pedreño, M.A.; García-Carmona, F.; Muñoz, R.Phytochem. Anal. 1998,9, 124–127.CrossRefGoogle Scholar
  11. [11]
    Wybraniec, S.; Platzner, I.; Geresh, S.; Gottlieb, H.E.; Haimberg, M.; Mogilnitzki, M.; Mizrahi, Y.Phytochemistry 2001,58, 1209–1212.CrossRefGoogle Scholar
  12. [12]
    Kobayashi, N.; Schmidt, J.; Nimtz, M.; Wray, V.; Schliemann, W.Phytochemistry 2000,54, 419–426.CrossRefGoogle Scholar
  13. [13]
    Cai, Y.Z.; Sun, M.; Wu, H.X.J. Agric. Food Chem. 1998,46, 2063–2070.CrossRefGoogle Scholar
  14. [14]
    Schwartz, S.J.; Von Elbe, J.H.J. Agric. Food Chem. 1980,28, 540–543.CrossRefGoogle Scholar
  15. [15]
    Strack, D.; Engel, U.; Wray, V.Phytochemistry 1987,26, 2399–2400.CrossRefGoogle Scholar
  16. [16]
    Forni, E.; Polesello, A.; Montefiori, D.; Maestrelli, A.J. Chromatogr. 1992,593, 177–183.CrossRefGoogle Scholar
  17. [17]
    Sobkowska, E.; Czapski, J.; Kaczmarek, R.Int. Food Ing. 1991,3, 24–28.Google Scholar
  18. [18]
    Stintzing, F.C.; Schieber, A.; Carle, R.Eur. Food. Res. Technol. 2001,212, 396–407.CrossRefGoogle Scholar
  19. [19]
    Krifa, F.; Villet, A.; Roussel, A.M.; Alary, J.Ann. Fals. Exp. Chim. 1987,928, 183–192.Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2002

Authors and Affiliations

  • J. A. Fernández-López
    • 1
  • R. Castellar
    • 1
  • J. M. Obón
    • 1
  • L. Almela
    • 2
  1. 1.Department of Chemical EngineeringPolytechnic University of CartagenaCartagenaSpain
  2. 2.Department of Agricultural ChemistryUniversity of Murcia, Campus of EspinardoMurciaSpain

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