, Volume 56, Issue 9–10, pp 545–551 | Cite as

Sensitive and rapid high-performance liquid chromatographic method for simultaneous determination of antidepressants in pharmaceutical formulations

  • J. J. Berzas
  • C. Guiberteau
  • A. M. Contento
  • V. Rodríguez
Originals Column Liquid Chromatography


An easy, rapid, and sensitive high-performance liquid chromatographic (HPLC) method is proposed for simultaneous determination of five antidepressants (trazodone, citalopram, fluoxetine, fluvoxamine and clomipramine) and norfluoxetine, a major metabolite of fluoxetine. Optimum conditions for the quantitative separation were investigated. The compounds were separated on a Nova Pack C18 column with acetonitrile-pH 2.5 phosphate buffer, 40∶60 (v/v), as mobile phase at a flow rate of 1.5 mL min−1. Stability of solutions, linearity, repeatability and reproducibility, accuracy, specificity, and detection and quantification limits were examined to validate the method. The method is rapid and sensitive and can be applied to the analysis of antidepressants in pharmaceutical formulations and biological fluids; norfluoxetine can also be determined in these matrices. We have tested the method by analysis of several pharmaceutical formations; recoveries obtained were between 102.7% and 98.2% of the labelled values.

Key Words

Column liquid chromatography Antidepressants Pharmaceutical formulations 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. [1]
    Berzas, J.J.; Villaseñor, M.J.; Contento, A.M.; Aguas, E.J. Chromatogr. Sci. 2000,38, 200.Google Scholar
  2. [2]
    Holister, L.E.Drugs 1981,22, 129.Google Scholar
  3. [3]
    Dugas, M.; Zarifian, E.; Leheuzey, M.F.; Rovei, V.; Durand, G.; Morselli, P.L.Ther. Drug Monit. 1980,2, 307.Google Scholar
  4. [4]
    Kasper, S.; Höflich, G.; Scholl, H.P.; Möller, H.J.Hum. Psychopharmacol. 1994,9, 1.CrossRefGoogle Scholar
  5. [5]
    Preskorn, S.H.J. Clin. Psychiatry 1994,55(Suppl. A), 6.Google Scholar
  6. [6]
    Lautz, R.I.; Farid, J.; Koons, K.Z.; Tenbargue, J.B.; Bopp, R.I.J. Chromatogr. B. Biomed. Appl. 1993,614(1), 175–179.CrossRefGoogle Scholar
  7. [7]
    Eap, C.B.; Gaillard, N.; Powell, K.; Baumann, P.J. Chromatogr. B. Biomed. Appl. 1996,682(2), 265–272.CrossRefGoogle Scholar
  8. [8]
    Eap, C.B.; Bouchoux, G.; Amey, M.; Cochard, N.; Savary, L.; Baumann, P.J. Chromatogr. Sci. 1998,36.Google Scholar
  9. [9]
    Meineke, I.; Schreeb, K.; Kress, I.; Gundert-Remy, U.Ther. Drug Monit. 1998,20(1), 14–19.CrossRefGoogle Scholar
  10. [10]
    Pichini, S.; Pacifini, R.; Altieri, I.; Pellegrini, M.; Zuccaro, P.J.Liq. Chromatogr. Related Technol. 1996,19(12), 1927–1935.Google Scholar
  11. [11]
    Torok-Both, G.A.; Baker, G.B.; Coutts, R.T.; Mackenna, K.F.; Aspeslet, L.I.J. Chromatogr. Biomed. Appl. 1992,579(1), 99–106.CrossRefGoogle Scholar
  12. [12]
    Alvarez, J.C.; Bothua, D.; Collignon, I.; Advenier, C.; Spreux-Varoquaux, O.J. Chromatogr. B. Biomed. Appl. 1998,707(1/2), 175–180.CrossRefGoogle Scholar
  13. [13]
    Wong, S.H.Y.; Dellafera, S.S.; Fernandes, R.; Kranzler, H.J. Chromatogr. 1990,499, 601.CrossRefGoogle Scholar
  14. [14]
    Raggi, M.A.; Mandrioli, R.; Casamenti, G.; Bugamelli, F.; Volterra, V.J. Pharm. Biomed. Anal. 1998,18(1/2), 193–199.CrossRefGoogle Scholar
  15. [15]
    Raggi, M.A.; Mandrioli, R.; Casamenti, G.; Volterra, V.; Desiderio, C.; Fanali, S.Chromatographia 1999,50(7/8), 423–427.CrossRefGoogle Scholar
  16. [16]
    Aymard, G.; Livi, P.; Pham, Y.T.; Diquet, B.J. Chromatogr. B 1997,700, 183.Google Scholar
  17. [17]
    Kristoffersen, L.; Bugge, A.; Lundanes, E.; Slordal, L.J. Chromatography B 1999,734, 229.Google Scholar
  18. [18]
    Lacassie, E.; Gaulier, J.M.; Marquet, P.; Rabatel, J.F.; Lachatre, G.J. Chromatogr. B 2000,742, 229.Google Scholar
  19. [19]
    Berzas, J.J.; Contento, A.M.; Villaseñor, M.J.; Aguas, E.Anal. Chim. Acta 2000,417, 169.CrossRefGoogle Scholar
  20. [20]
    Desiderio, C.; Rudaz, S.; Raggli, M.A.; Fanali, S.Electrophoresis 1999,20(17), 3432.CrossRefGoogle Scholar
  21. [21]
    Berzas, J.J.; Contento, A.M.Chromatographia 2002,55, 369–373.CrossRefGoogle Scholar
  22. [22]
    Goodnough, D.B.; Baker, G.B.; Coutts, R.T.J. Pharmacol. Toxicol. Methods 1995,34, 143.CrossRefGoogle Scholar
  23. [23]
    Berzas, J.J.; Contento, A.M.; Villaseñor, M.J.J. Chromatogr. B. 2002,769, 261–268.CrossRefGoogle Scholar
  24. [24]
    Storch de Gracia, J.J.; Ed. Alhambra,1987, pp. 122.Google Scholar
  25. [25]
    Lark, P.D.; Craven, B.R.; Bosworth, R.C.L.The Handling of Chemical Data, Pergamon Press, Oxford,1968, Chapter 4, pp. 136–206.Google Scholar
  26. [26]
    Massart, D.L.; Vandeginste, B.G.M.; Deming, S.N.; Kaufmann, L. In:Chemometrics: A Textbook, 1st edn, Vandeginste, B.G.M.; Kaufmann, L., Eds, Oxford University Press, Oxford,1988.Google Scholar
  27. [27]
    Fabre, H.; Le Bris, A.; Balchin, M.D.J. Chromatogr. A 1995,697, 81.CrossRefGoogle Scholar
  28. [28]
    Huber, L.Applications of Diode-Array Experience, Hewlett-Packard Publication no. 12-5953-2330,1989.Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2002

Authors and Affiliations

  • J. J. Berzas
    • 1
  • C. Guiberteau
    • 1
  • A. M. Contento
    • 1
  • V. Rodríguez
    • 1
  1. 1.Department of Analytical Chemistry and Food TechnologyUniversity of Castilla-La ManchaCiudad RealSpain

Personalised recommendations