Fabrication and characterization of a novel biporous spherical adsorbent for protein chromatography

Summary

A novel rigid spherical biporous poly(glycidyl methacrylate-triallyl isocyanurate-divinylbenzene) resin (denoted Resin B) has been fabricated by radical suspension copolymerization, with superfine granules of calcium carbonate, and toluene andn-heptane, as porogenic agents, as the basis of a novel porogenic mode, cooperation of solid granules and solvents. The pore structure, static adsorption behavior, and chromatographic properties of Resin B were characterized and compared with those of Resin A (with only solvents as porogens). The results indicated that the biporous resin is a promising chromatographic medium for highspeed protein separation with good mechanical performance and high dynamic adsorption capacity and column efficiency at high flow rates.

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Shi, Y., Sun, Y. Fabrication and characterization of a novel biporous spherical adsorbent for protein chromatography. Chromatographia 57, 29–35 (2003). https://doi.org/10.1007/BF02497474

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Key Words

  • Column liquid chromatography
  • Biporous matrix
  • Radical suspension copolymerization
  • Proteins