Fabrication and characterization of a novel biporous spherical adsorbent for protein chromatography


A novel rigid spherical biporous poly(glycidyl methacrylate-triallyl isocyanurate-divinylbenzene) resin (denoted Resin B) has been fabricated by radical suspension copolymerization, with superfine granules of calcium carbonate, and toluene andn-heptane, as porogenic agents, as the basis of a novel porogenic mode, cooperation of solid granules and solvents. The pore structure, static adsorption behavior, and chromatographic properties of Resin B were characterized and compared with those of Resin A (with only solvents as porogens). The results indicated that the biporous resin is a promising chromatographic medium for highspeed protein separation with good mechanical performance and high dynamic adsorption capacity and column efficiency at high flow rates.

This is a preview of subscription content, log in to check access.


  1. [1]

    Zeng, X.; Ruckenstein, E.J. Membrane Sci. 1998,148, 195–205.

    CAS  Article  Google Scholar 

  2. [2]

    Garcia, M.C.; Marina, M.L.; Torre, M.J. Chromatogr. A 2000,880, 169–187.

    CAS  Article  Google Scholar 

  3. [3]

    Horváth, J.; Boschetti, E.; Guerrier, L.; Cooke, N.J. Chromatogr. A 1994,679, 11–22.

    Article  Google Scholar 

  4. [4]

    Tennikov, M.B.; Gazdina, N.V.; Tennikova, T.B.; Svec, F.J. Chromatogr. A 1998,798, 55–64.

    CAS  Article  Google Scholar 

  5. [5]

    Afeyan, N.B.; Gordon, N.F.; Mazasaroff, I.; Varady, L.; Fulton, S.P.; Yang, Y.B.; Regnier, F.E.J. Chromatogr. 1990,519, 1–29.

    CAS  Article  Google Scholar 

  6. [6]

    Carta, G.; Rodrigues, A.E.Chem. Eng. Sci. 1993,48, 3927–3935.

    CAS  Article  Google Scholar 

  7. [7]

    Frey, D.; Schwesnheim, E.; Horváth, Cs.Biotechnol. Prog. 1993,9, 93–100.

    Article  Google Scholar 

  8. [8]

    Zhang, M.L.; Sun, Y.J. Chromatogr. A 2001,922, 77–86.

    CAS  Article  Google Scholar 

  9. [9]

    Shi, Y.; Dong, X.-Y.; Sun, Y.Chromatographia 2002,55(7/8), 405–410.

    CAS  Article  Google Scholar 

  10. [10]

    Yu, Y.H.; Sun, Y.J. Chromatogr. A 1999,855, 129–136.

    CAS  Article  Google Scholar 

  11. [11]

    Zhou, X.; Xue, B.; Sun, Y.Biotechnol. Prog. 2001,17, 1093–1098.

    CAS  Article  Google Scholar 

  12. [12]

    Wu, G.; Brown, G.R.React. Polym. 1991,14, 49–61.

    CAS  Article  Google Scholar 

  13. [13]

    He, L.Z.; Gan, Y.R.; Sun, Y.Bioprocess Eng. 1997,17(5), 301–305.

    CAS  Article  Google Scholar 

  14. [14]

    Kegbubgerm, A.L.Principles of Biochemistry, Worth, New York,1982.

    Google Scholar 

  15. [15]

    Guyot, A.; Bartholin, M.Prog. Polym. Sci. 1982,8, 277–331.

    CAS  Article  Google Scholar 

  16. [16]

    Nash, D.C.; Chase, H.A.J. Chromatogr. A 1998,807, 185–207.

    CAS  Article  Google Scholar 

  17. [17]

    Boyer, P.M.; Hsu, J.T.AIChE J. 1992,38, 259–272.

    CAS  Article  Google Scholar 

  18. [18]

    Levison, P.R.; Mumford, C.; Streater, M.; Brandt-Nielsen, A.; Pathirana, N.D.; Badger, S.E.J. Chromatogr. A 1997,760, 151–158.

    CAS  Article  Google Scholar 

  19. [19]

    Van Deemter, J.J.; Zuiderweg, F.J.; Klinkenberg, A.Chem. Eng. Sci. 1956,5, 271–289.

    Article  Google Scholar 

  20. [20]

    Deen, W.M.AIChE J. 1987,33, 1409–1425.

    CAS  Article  Google Scholar 

  21. [21]

    Nash, D.C.; Chase, H.A.J. Chromatogr. A 1997,776, 65–73.

    CAS  Article  Google Scholar 

  22. [22]

    Staby, A.; Jensen, I.H.; Mollerup, I.J. Chromatogr. A 2000,897, 99–111.

    CAS  Article  Google Scholar 

  23. [23]

    Gowanlock, D.; Bailey, R.; Cantwell, F.F.J. Chromatogr. A 1996,726, 1–23.

    CAS  Article  Google Scholar 

  24. [24]

    Yang, Y.B.; Harrison, K.; Kindsvater, J.J. Chromatogr. A 1996,723, 1–10.

    CAS  Article  Google Scholar 

  25. [25]

    Rendueles de la Vega, M.; Chenou, C.; Loureiro, J.M.; Rodrigues, A.E.Biochem. Eng. J. 1998,1, 11–23.

    Article  Google Scholar 

  26. [26]

    Afeyan, N.B.; Fulton, S.P.; Regnier, F.E.J. Chromatogr. 1991,544, 267–279.

    CAS  Article  Google Scholar 

  27. [27]

    Liapis, I.; McCoy, M.A.J. Chromatogr. 1992,599, 87–104.

    CAS  Article  Google Scholar 

  28. [28]

    Rodrigues, A.E.J. Chromatogr. B 1997,699, 47–61.

    CAS  Google Scholar 

  29. [29]

    Koh, J.-H.; Guiochon, G.J. Chromatogr. A 1998,796, 41–57.

    CAS  Article  Google Scholar 

Download references

Author information



Rights and permissions

Reprints and Permissions

About this article

Cite this article

Shi, Y., Sun, Y. Fabrication and characterization of a novel biporous spherical adsorbent for protein chromatography. Chromatographia 57, 29–35 (2003). https://doi.org/10.1007/BF02497474

Download citation

Key Words

  • Column liquid chromatography
  • Biporous matrix
  • Radical suspension copolymerization
  • Proteins