Advertisement

Chromatographia

, Volume 46, Issue 3–4, pp 141–144 | Cite as

Analysis of 5-hydroxy-N-methyl-2-pyrrolidone and 2-hydroxy-N-methylsuccinimide in plasma

  • B. A. G. Jönsson
  • B. Åkesson
Originals

Summary

A method for the determination of 5-hydroxy-N-methyl-2-pyrrolidone (5-HNMP) and 2-hydroxy-N-methylsuccinimide (2-HMSI) in plasma was developed. 5-HNMP and 2-HMSI are metabolites to the widely used organic solvent N-methyl-2pyrrolidone (NMP). The 5-HNMP and 2-HMSI were purified from plasma by C8 solid phase extraction, derivatised by bistrimethylsilyl trifluoroacetamid, and analysed by gas chromatography with mass spectrometric detection. For 5-HNMP, the precision was 2–7 % (120 and 780 ng mL−1) and the detection limit was 6 ng mL−1 (m/z 98). For 2-HMSI, the precision was 2–9 % (160 and 1000 ng mL−1) and the detection limit was 4 ng mL−1 (m/z 144). The method is applicable for analysis of plasma samples from workers exposed to NMP.

Key Words

Gas chromatography-mass spectrometry Biological monitoring 5-hydroxy-N-methyl-2-pyrrolidone 2-hydroxy-N-methylsuccinimide Plasma samples 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    B. Åkesson, Arbete Halsa40, 1 (1994).Google Scholar
  2. [2]
    J. Priborsky, E. Mühlbachova J. Pharm. Pharmacol.42, 468 (1990).Google Scholar
  3. [3]
    P. J. Becci, M. J. Knickerbocker, E. L. Reagan, R. A. Parent, L. W. Burnette, Fundam. Appl. Toxicol.2, 273 (1982).Google Scholar
  4. [4]
    U. Hass, S. P. Lund, J. Elsner, Neurotoxicol. Teratol.16, 241 (1994).CrossRefGoogle Scholar
  5. [5]
    G. M. Solomon, E. P. Morse, M. J. Garbo, D. K. Milton, J. Occup. Environ Med.38, 705 (1996).CrossRefGoogle Scholar
  6. [6]
    H. Blome, M. Hennig, Staub-Reinhalt Luft44, 27 (1984).Google Scholar
  7. [7]
    B. Åkesson, K. Paulsson, Occup. Environ. Med.54, 236 (1997).CrossRefGoogle Scholar
  8. [8]
    C. Ursin, C. M. Hansen, J. W. Van Dyk, P. O. Jensen, I. J. Christensen, J Ebbehoej, Am. Ind. Hyg. Assoc. J.56, 651 (1995).Google Scholar
  9. [9]
    B. Åkesson, B. A. G. Jönsson, Drug Metab. Dispos.25, 267 (1997).Google Scholar
  10. [10]
    B. A. G. Jönsson, B. Åkesson, J. Chromatogr. submitted for publication.Google Scholar
  11. [11]
    B. A. G. Jönsson, B. Åkesson, J. Chromatogr. in press.Google Scholar
  12. [12]
    J. C. Miller, J. N. Miller, Statistics for Analytical Chemistry, Ellis Horwood, Chichester 1984.Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1997

Authors and Affiliations

  • B. A. G. Jönsson
    • 1
  • B. Åkesson
    • 1
  1. 1.Department of Occupational and Environmental Medicine, Institute of Laboratory MedicineUniversity HospitalLundSweden

Personalised recommendations