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Russian Chemical Bulletin

, Volume 49, Issue 8, pp 1464–1467 | Cite as

Oxygen effects in aqueous solutions of laccases: Absorption spectra and reactivity

  • A. A. Revina
  • G. I. Khaikin
  • E. L. Protasova
  • N. B. Mezentseva
  • E. V. Stepanova
  • O. V. Skorobogat'ko
Chemistry of Natural Compounds and Bioorganic Chemistry

Abstract

The optical absorption spectra of laccases in aqueous solutions were found to undergo reversible changes in the presence of O2. It was suggested that dioxygen is coordinated in the active center of the completely oxidized native enzyme. Abnormal behavior of superoxide radical anions upon variation of the laccase concentration was found by pulse radiolysis.

Key words

laccases molecular oxygen electron transfer optical absorption spectrum pulse radiolysis superoxide radical anion 

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Copyright information

© Kluwer Academic/Plenum Publishers 2000

Authors and Affiliations

  • A. A. Revina
    • 1
  • G. I. Khaikin
    • 1
  • E. L. Protasova
    • 1
  • N. B. Mezentseva
    • 2
  • E. V. Stepanova
    • 1
  • O. V. Skorobogat'ko
    • 2
  1. 1.A. N. Frumkin Institute of ElectrochemistryRussian Academy of SciencesMoscowRussian Federation
  2. 2.A. N. Bach Institute of BiochemistryRussian Academy of SciencesMoscowRussian Federation

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