, Volume 56, Issue 1, pp S165–S168 | Cite as

Determination of neutral cannabinoids in hemp samples by overpressured-layer chromatography

  • B. Szabady
  • E. Hidvégi
  • Sz. Nyiredy
Originals Overpressed Layer Chromatography


The overpressured-layer chromatographic separation of neutral cannabinaids (Δ9 -tetrahydro-cannabinol, cannabidiol, cannabinol, cannabigerol and cannabichromene) has been achieved on amino HPTLC plates with dichloromethane as mobile phase. By use of bidirectional development up to 30 samples can be analysed on a 10 cm×20 cm plate within 4 min. Evaluation was performed by slit-scanning densitometry at 200 nm. System-suitability data confirm the applicability of the method.

Key Words

Overpressured-layer chromatography (OPLC) Neutral cannabinoids Amino-modified silica Cannabis 


  1. [1]
    Szabady, B.; Ruszinkó, M.; Nyiredy, Sz.J. Planar Chromatogr. 1995,8, 279–283.Google Scholar
  2. [2]
    Smith, R. M.J. Forensic Sci. 1997,42, 610–618.Google Scholar
  3. [3]
    Lavanya, K. L.; Baggi, T. R.Forensic Sci. Int. 1990,47, 165–171.CrossRefGoogle Scholar
  4. [4]
    Wagner, H.; Bladt, S.Plant Drug Analysis-A Thin Layer Chromatographic Atlas, Springer, Berlin,1996.Google Scholar
  5. [5]
    Hughes, R. B.; Kessler, R. R.J. Forensic Sci. 1979,24, 842–846.Google Scholar
  6. [6]
    Mali, B. D.; Parulekar, P. P.J. Chromatogr. 1988,457, 383–386.CrossRefGoogle Scholar
  7. [7]
    Pranitis, P. A. F.; Kornfield, J. M.J. Chromatogr. 1982,247, 197–200.CrossRefGoogle Scholar
  8. [8]
    Szulc, C.; Zarzecka, R.; Szuchnik, A.Forensic Sci. Int. 1990,46, 117–120.CrossRefGoogle Scholar
  9. [9]
    Szabady, B.; Ruszinkó, M.; Nyiredy, Sz.Chromatographia 1997,45, 369–372.Google Scholar
  10. [10]
    Macherey-Nagel Application Note, A-337,2000.Google Scholar
  11. [11]
    Szabady, B.; Fatér, Zs.; Nyiredy, Sz.J. Planar Chromatogr. 1999,12, 82–85.Google Scholar
  12. [12]
    Smith, R. M.J. Forensic Sci. 1997,42, 610–618.Google Scholar
  13. [13]
    Maseda, C.; Fukui, Y.; Kimura, K.; Matsubara, K.J. Forensic Sci. 1983,28, 911–921.Google Scholar
  14. [14]
    Vollner, L.; Bieniek, D.; Korte, F.Regul. Toxicol. Pharmacol. 1986,6, 348–358.CrossRefGoogle Scholar
  15. [15]
    Harvey, D. J. InAnalytical Methods in Human Toxicology: Curry, A. S., Ed., VCH,1985.Google Scholar
  16. [16]
    Oroszlán, P.; Verzár-Petri, G.; Mincsovics, E.; Székely, T.J. Chromatogr. 1987.388, 217–224.CrossRefGoogle Scholar
  17. [17]
    Pothier, J.; Galand, N.; Viel, C.J. Chromatogr. 1993,634, 356–359.CrossRefGoogle Scholar
  18. [18]
    Jork, H.; Funk, W.; Fischer, W.; Wimmer, H.Thin-Layer Chromatography, Reagents and Detection Methods, Vol. 1a, VCH, Weinheim,1991,Google Scholar
  19. [19]
    Hidvégi, E.; Perneczki, S.; Forstner, M.J. Planar Chromatogr. 2000,13, 414–419.Google Scholar
  20. [20]
    Ferenczi-Foder, K.; Végh, Z.; Nagy-Turák, A.; Renger, B.; Zeller, M. InPlanar Chromatography—A Retrospective View for the Third Millennium: Nyiredy, Sz., Ed Springer Budapest,2001.Google Scholar
  21. [21]
    Recommended Methods for the Detection and Assay of Heroin, Cannabinoids, Cocaine, Amphetanime, Methamphetamine and Ring-Substituted Amphetamine Derivatives in Biological Specimens, United Nations, New York,1995.Google Scholar
  22. [22]
    Richter, DToxichem+Krimtech 2000,67, 57–59.Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2002

Authors and Affiliations

  • B. Szabady
    • 1
  • E. Hidvégi
    • 2
  • Sz. Nyiredy
    • 1
  1. 1.Research Institute for Medicinal PlantsBudakalászHungary
  2. 2.National Insitute of Forensic ToxicologyBudapest 70Hungary

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