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Chromatographia

, Volume 56, Issue 1, pp S91–S94 | Cite as

Application of pseudo-reversed-phase systems to the purification and isolation of biologically active taxoids from plant material

  • M. L. Hajnos
  • K. Glowniak
  • M. Waksmundzka-Hajnos
  • S. Piasecka
Originals Column Liquid Chromatography

Summary

Silica and aqueous methanolic mobile phases have been optimized for the purification of crude yew extracts. From the recoveries measured for 10-deacetylbaccatin Ill and paclitaxel it was found that 20% methanol in water elutes all taxoids from the adsorbent and that non-polar materials remain in the starting band; this enables preparation of the samples before HPLC analysis. Partial separation of the taxoid fraction was achieved by elution with pure water then 6:4 methanol-water. Such systems on a large scale can be used for preparative separation of taxoids. Partially separated fractions are re-chromatographed in a normal-phase system consisting of silica with a non-aqueous mobile phase. This procedure enables isolation of 10-deacetylbaccatin Ill from the fraction.

Key Words

Column liquid chromatography Taxoids Silica with aqueous mobile phases Purification Isolation DrylabG software 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2002

Authors and Affiliations

  • M. L. Hajnos
    • 1
  • K. Glowniak
    • 1
  • M. Waksmundzka-Hajnos
    • 2
  • S. Piasecka
    • 1
  1. 1.Department of PharmacognosyMedical UniversityLublinPoland
  2. 2.Department of Inorganic and Analytical ChemistryMedical UniversityLublinPoland

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