, Volume 56, Issue 1, pp S31–S39 | Cite as

Phenomenological approach to the mechanism of retention in RP HPLC

  • L. Szepesy
Originals Column Liquid Chromatography


The retention process in reversed-phase liquid chromatography (RPLC) and the mechanism behind it can best be followed by a phenomenological description of molecular interactions. This can be accomplished by using the linear solvation-energy relationship (LSER). The retention factor (logk) can be expressed as the difference between the sum of the positive, retention-increasing, terms and the sum of the negative, retention-reducing, terms of the LSER equation. Calculated and measured logk values were compared for five columns with widely different characteristics. From among the 32 test solutes used ten with significantly different solvation properties were selected to demonstrate the effect of the molecular properties of the solutes on the mechanism of retention. The effect of mobile-phase composition on the retention process was also demonstrated. The relative contributions of the different molecular interactions to the mechanism of retention was illustrated by comparing the percentage contributions of the individual terms of the LSER equation.

Key Words

Column liquid chromatography Molecular interactions Linear solvation-energy relationships Effect of molecular properties of the solutes 


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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2002

Authors and Affiliations

  • L. Szepesy
    • 1
  1. 1.Department of Chemical TechnologyBudapest University of Technology and EconomicsBudapestHungary

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