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Optimization of an HPLC method for the separation of erythromycin and related compounds using factorial design

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Summary

An HPLC method based on a prodigy ODS2 column with a mobile phase contianging phosphate buffer, tetrabutylammonium hydrogen sulfate (TBA) and an acetonitrile gradient was optimized for the determination of erythromycin in delayed-release capsules. A central composite face-centered (CCF) factorial design was used with the factors pH, concentration of TBA and gradient slope. The method could separate erythromycin A from 7 related erythromycins.

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Authors and Affiliations

  1. Pharmaceutical Analysis, AstraZeneca R&D Södertälje, 15185, Södertälje, Sweden

    V. Harang

  2. Dept. of Analytical Pharmaceutical Chemistry, Biomedical Centre, University of Uppsala, Box 574, 751 23, Uppsala, Sweden

    D. Westerlund

Authors
  1. V. Harang
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  2. D. Westerlund
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Harang, V., Westerlund, D. Optimization of an HPLC method for the separation of erythromycin and related compounds using factorial design. Chromatographia 50, 525–531 (1999). https://doi.org/10.1007/BF02493656

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  • DOI: https://doi.org/10.1007/BF02493656

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