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Simultaneous determination of ursodeoxycholic acid and its glycine-conjugate in serum as phenacyl esters using multidimensional liquid chromatography

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Summary

A narrowbore high-performance liquid chromatographic (HPLC) method using column switching is described for the simultaneous determination of ursodeoxycholic acid (UDCA) and glyco-UDCA (GUDCA) from serum samples as their phenacyl esters. Serum samples were subjected to a preliminary clean-up using octadecylsilane reversed-phase extraction and derivatized with phenacylbromide. The purification, fractionation and concentration of UDCA and GUDCA from the esterified serum sample were performed on-line by appropriate switching of columns. Limit of detection (LOD) of UDCA and GUDCA were 5 ng and the absolute mean recoveries averaged 84.4±8.2% and 85.2±8.4%, respectively. This method was successfully applied to the pharmacokinetic study of UDCA in rats and human.

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Authors and Affiliations

  1. College of Pharmacy, Wonkwang University, 570-749, Iksan, Korea

    S. J. Choi, C. K. Jeong, H. M. Lee & H. S. Lee

  2. Toxicology Research Center, Korea Research Institute of Chemical Technology, 305-606, Taejeon, Korea

    K. Kim

  3. Taejeon Health and Environment Research Institute, 305-153, Taejeon, Korea

    K. S. Do

Authors
  1. S. J. Choi
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  2. C. K. Jeong
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  3. H. M. Lee
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  4. K. Kim
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  5. K. S. Do
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  6. H. S. Lee
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Choi, S.J., Jeong, C.K., Lee, H.M. et al. Simultaneous determination of ursodeoxycholic acid and its glycine-conjugate in serum as phenacyl esters using multidimensional liquid chromatography. Chromatographia 50, 96–100 (1999). https://doi.org/10.1007/BF02493624

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  • DOI: https://doi.org/10.1007/BF02493624

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