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Requirement of alkaline hydrolysis for estimating chloroplast RNA using the orcinol reaction

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Abstract

Considerable amounts of orcinol-reacting material other than RNA-ribose were extracted with hot PCA from isolated spinach and broad bean chloroplasts, while cold PCA only incompletely extracted the chloroplast RNA. The procedure utilizing alkaline hydrolysis followed by an orcinol test was found to be appropriate for measuring the chloroplast RNA.

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Abbreviations

PCA:

perchloric acid

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Authors and Affiliations

  1. Department of Biology, Faculty of General Education, Tokyo University of Agriculture and Technology, Fuchu, 183, Tokyo

    Takeshi Nitta

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  1. Takeshi Nitta
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Nitta, T. Requirement of alkaline hydrolysis for estimating chloroplast RNA using the orcinol reaction. Bot Mag Tokyo 92, 145–149 (1979). https://doi.org/10.1007/BF02493388

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  • DOI: https://doi.org/10.1007/BF02493388

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