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Isolation and culture of protoplasts from moss protonemata

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Abstract

Protoplats were enzymatically prepared from protonemata of the mossPlagiomnium vesicatum, which was cultured on a Murashige-Skoog's medium supplemented with 0.2% yeast extract and solidified with agar. The protoplasts incubated in soft agar medium containing sucrose began to divide after 15 days of incubation, and after 50 days 10- to 15-cell aggregates were present. When transferred to a sugar-free medium, the cells in the aggregates grew into filaments.

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Authors and Affiliations

  1. Biological Laboratory, Faculty of Education, Fukui University, 910, Fukui

    Masuo Maeda

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  1. Masuo Maeda
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Maeda, M. Isolation and culture of protoplasts from moss protonemata. Bot Mag Tokyo 92, 105–110 (1979). https://doi.org/10.1007/BF02493383

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  • DOI: https://doi.org/10.1007/BF02493383

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