, Volume 55, Supplement 1, pp S193–S194 | Cite as

Measurement of plasma tyrosine by HPLC-UV or LC-MS-MS for assessing chemically induced tyrosinaemia

  • P. C. Holme
  • K. A. Woods


Certain chemicals can cause a marked increase in the levels of the amino acid tyrosine in plasma (tyrosinaemia). An HPLC-UV method involving the removal of plasma proteins using a simple ultrafiltration device was developed here and has been in use for a number of years. This has now been adapted to LC-MS-MS and this has resulted in great improvements in productivity. Validation data are presented for both methods over the relevant range of 5–500 μg mL−1 Although a low level of detection is not required, HPLC-MS-MS analysis presents a great advantage in sample throughput and improved cost effectiveness with no obvious disadvantages.

Key Words

Column liquid chromatography LC-MS-MS UV Tyrosine 

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2002

Authors and Affiliations

  • P. C. Holme
    • 1
  • K. A. Woods
    • 1
  1. 1.Syngenta CTLMacclesfieldUK

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