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Optimization of the reversed-phase high-performance liquid chromatographic separation of the enantimers of a cationic chiral drug (tolperisone) on a heptakis(6-azido-6-deoxy) perphenylcarbamated β-cyclodextrin column

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Summary

Heptakis(6-azido-6-deoxy) perphenylcarbamated β-cyclodextrin has been synthesized and chemically immobilized on silica gel for use as a chiral stationary phase (PC-CSP) for analytical separation of the enantiomers of chiral drugs. Separation of the enantiomers of tolperisone was studied by high-performance liquid chromatography under reversed-phase conditions. The chromatographic conditions were optimized by varying mobile phase pH, composition, ionic strength, and velocity; 40:60 methanol-1% triethylammonium acetate (TEAA) buffer, pH 5.5, was found to be the most suitable for this separation.

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Authors and Affiliations

  1. Department of Chemical and Environmental Engineering, National University of Singapore, 4 Engineering Drive 4, 117576, Singapore

    T. Velmurugan & C. B. Ching

  2. Department of Chemistry, National University of Singapore, 3 Science Drive 3, 117543, Singapore

    S. C. Ng

  3. Chemical and Process Engineering Centre, National University of Singapore, 4 Engineering Drive 4, 117576, Singapore

    C. B. Ching, Z. W. Bai & T. T. Ong

Authors
  1. T. Velmurugan
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  2. C. B. Ching
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  3. S. C. Ng
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  4. Z. W. Bai
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  5. T. T. Ong
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Velmurugan, T., Ching, C.B., Ng, S.C. et al. Optimization of the reversed-phase high-performance liquid chromatographic separation of the enantimers of a cationic chiral drug (tolperisone) on a heptakis(6-azido-6-deoxy) perphenylcarbamated β-cyclodextrin column. Chromatographia 56, 229–232 (2002). https://doi.org/10.1007/BF02493217

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  • DOI: https://doi.org/10.1007/BF02493217

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