, Volume 56, Issue 3–4, pp 175–180 | Cite as

Ethylbenzene-modified fused-silica columns for capillary electrophoresis

  • M. V. Russo
Originals Electrophoresis


Coated capillaries modified with a hydrocarbon layer have been developed. Modification of the surface with ethylbenzene greatly improved the electrophoretic performance of the capillaries. The column efficiency for basic organic compounds was as high as 378000 theoretical plates per meter on a 50 μm i.d. ethylbenzene-surface-treated fused-silica capillary column. This value did not change during 25 replicate analyses and the capillary columns were very stable against continuous treatment for 30 h with buffer of pH 10 and treatment for 3 h with HCl of pH 1 and NaOH of pH 12. The relative standard deviation of run-to-run, day-to-day, and capillary-to-capillary for coating with the ethylbenzene layer was<2.6%, and reproducibility was good. The separation of four aromatic amines and six pharmacological amines at pH 2.5 is reported.

Key Words

Capillary electrophoresis Surface modification Ethylbenzene coating Ethylbenzene-n-hydrocarbon coating Basic organic compounds 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. [1]
    Hjerten, S.J. Chromatogr. 1985,347, 191.CrossRefGoogle Scholar
  2. [2]
    McCormik, R.M.Anal. Chem. 1988,60, 2322.CrossRefGoogle Scholar
  3. [3]
    Cobb, K.A.; Dolnik, V.; Novotny, M.Anal. Chem. 1990,62, 2478.CrossRefGoogle Scholar
  4. [4]
    Huang, M.; Vorkink, W.P.; Lee, M.L.J. Microcolumn Sep. 1992,4, 233.CrossRefGoogle Scholar
  5. [5]
    Huang, M.; Lee, M.L.J. Microcolumn Sep. 1992,4, 491.CrossRefGoogle Scholar
  6. [6]
    Sagiv, J.J. Am. Chem. Soc. 1980,102, 92.CrossRefGoogle Scholar
  7. [7]
    Amador, S.M.; Pachence, J.M.; Fishetti, R.; McCauley, J.P. Jr.; Smith, A.B. III: Blasie, J.K.Langmuir 1993,9, 812.CrossRefGoogle Scholar
  8. [8]
    Ohtake, T.; Mino, N.; Ogawa, K.Langmuir 1992,8, 2081.CrossRefGoogle Scholar
  9. [9]
    Brandriss, S.; Margel, S.Langmuir 1993,9, 1232.CrossRefGoogle Scholar
  10. [10]
    Wirth, M.J.; Fatunmbi, H.O.Anal. Chem. 1993,65, 822.CrossRefGoogle Scholar
  11. [11]
    Huang, M.; Dubrovcakova-Schneiderman, E.; Novotny, M.V.; Fatunmbi, H.O.; Wirth, M.J.J. Microcolumn Sep. 1993,6, 571.CrossRefGoogle Scholar
  12. [12]
    Katayama, H.; Ishihama, Y.; Asakawa, N.Anal. Chem. 1998,70, 5272.CrossRefGoogle Scholar
  13. [13]
    Huang, X.; Horvath, C.J. Chromatogr. A 1997,788, 155.CrossRefGoogle Scholar
  14. [14]
    Izumi, T.; Nagahoi, T.; Okuyama, T.J. High Resol. Chromatogr. 1991,14, 351.CrossRefGoogle Scholar
  15. [15]
    Nielen, M.W.F.J. High Resol. Chromatogr. 1993,16, 62.CrossRefGoogle Scholar
  16. [16]
    Liu, P.Z.; Malik, A.; Kuchar, M.C.J.; Vorkink, W.P.; Lee, M.I.J. Microcolumn Sep. 1993,5, 245.CrossRefGoogle Scholar
  17. [17]
    Liu, P.Z.; Malik, A.; Kucar, M.C.; Lee, M.L.J. Microcolumn Sep. 1994,6, 581.CrossRefGoogle Scholar
  18. [18]
    Zhao, Z.; Malik, A.; Lee, M.L.Anal. Chem. 1993,65, 2747.CrossRefGoogle Scholar
  19. [19]
    Adamson, A.W.Physical Chemistry of Surfaces, 4th edn, Wiley, New York,1982.Google Scholar
  20. [20]
    Bard, A.; Faulkner, L.Electrochemical Methods: Fundamentals and Applications, Wiley, NY,1980.Google Scholar
  21. [21]
    Morrison, R.T.; Boyd, R.N.Chimica Organica, 5th edn, Casa Editrice Ambrosiana, Milano,1991.Google Scholar
  22. [22]
    Vogel, A.I.Chimica Organica Pratica, 3rd edn, Casa Editrice Ambrosiana, Milano,1967.Google Scholar
  23. [23]
    Jorgenson, J.W.; Lukacs, K.D.J. Chromatogr. 1981,218, 209.CrossRefGoogle Scholar
  24. [24]
    Jorgenson, J.W.; Lukacs, K.D.Anal. Chem. 1981,53, 1298.Google Scholar
  25. [25]
    Russo, M.V.; Goretti, G.,J. Chromatogr. A 2000,871, 279.CrossRefGoogle Scholar

Copyright information

© Friedr Vieweg & Sohn Verlagsgesellschaft mbH 2002

Authors and Affiliations

  • M. V. Russo
    • 1
  1. 1.Facoltà di Agraria (DiSTAAM)Università del Molise, Via De SanctisCampobasso (CB)Italy

Personalised recommendations