Summary
A sensitive GC-MS method was developed for the determination of a new retinoic acid metabolism blocking agent R115866 in human plasma samples. A deuterium labeled internal standard (i.s.) was used. Sample preparation consisted of a one step liquid-liquid extraction, followed by derivatisation with pentafluorobenzylbromide and a clean-up step. The linearity, extraction recovery, accuracy and precision of the method were tested, as well as the stability of the compound. The calibration range was from 0.01 to 10 ng mL−1 and the LLOQ was 0.01 ng mL−1. The method was successfully applied for the determination of unchanged R115866 plasma levels after oral dosing in healthy volunteers.
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Verhaeghe, T., Diels, L. Determination of the retinoic acid metabolism blocking agent R115866 in human plasma by gas chromatography-high resolution mass spectrometry. Chromatographia 52 (Suppl 1), S109–S112 (2000). https://doi.org/10.1007/BF02493140
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DOI: https://doi.org/10.1007/BF02493140