, Volume 52, Supplement 1, pp S55–S56 | Cite as

Use of non-ambient temperatures in separatory runs

Assay Acceptability and Strategies


Some assay publications cited in past volumes of this Series allude to use, especially with RP-packings or CSP's, of a non-ambient temperature; but irritatingly, few indicate why. Part II gives further literature and reinforces the view that there may be bioanalytical benefit, especially in respect of resolution or intererants, from empirical trial of unorthodox temperatures. It is vital that such trials be adequately reported.

Key Words

HPLC column heating Enantiomer separation, temperature influence Resolution improvement in HPLC Packings, temperature-dependent behaviour Temperature choice for HPLC separations 

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2000

Authors and Affiliations

  • E. Reid
    • 1
  1. 1.Guildford Academic AssociatesGuildfordUK

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