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Chromatographia

, Volume 53, Issue 11–12, pp 641–644 | Cite as

HPLC quantification of carvedilol in small plasma volumes from children

  • F. Behn
  • S. Läer
  • T. S. Mir
  • H. Scholz
Originals Column Liquid Chromatography

Summary

A sensitive high-performance liquid chromatographic method has been developed for the determination of the β-receptor blocker carvedilol in small volumes of plasma from pediatric patients. Carvedilol and the internal standard naftopidil were extracted from only 300 μL plasma with solid-phase extraction columns and eluted with a mixture of 80% (v/v) acetonitrile and 20% (v/v) potassium acetate buffer (0.1 M, pH 5). Analysis of the extracts was performed on a Spherisorb C6 column with a mobile phase of 65% acetonitrile, 35% potassium acetate buffer (0.25 M, pH 4) and fluorescence detection. Carvedilol and the internal standard showed recoveries of 87.0±13.9% and 97.7±18.0% respectively (n=75, each; values are means±standard deviations). The analysis of carvedilol was linear over a range of 0.40–242 ng mL−1, the limit of quantification was 0.40 ng mL−1. As an application of the assay, a profile of plasma concentrations versus time of a six-month-old patient is reported.

Key Words

Column liquid chromatography Solid phase extraction Carvedilol Plasma from Children 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2001

Authors and Affiliations

  • F. Behn
    • 1
  • S. Läer
    • 1
  • T. S. Mir
    • 2
  • H. Scholz
    • 1
  1. 1.Institut für Experimentelle und Klinische Pharmakologie und Toxikologie Abteilung für PharmakologieUniversitätsklinikum Hamburg-EppendorfHamburgGermany
  2. 2.Klinik und Poliklinik für Kinder- und Jugendmedizin, Abteilung für KinderkardiologieUniversitätsklinikum Hamburg-EppendorfHamburgGermany

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