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Chromatographia

, Volume 53, Issue 11–12, pp 629–634 | Cite as

Stability—Indicating assay for haloperidol syrup by high-performance liquid chromatography

  • R. Driouich
  • H. Trabelsi
  • K. A. Bouzouita
Originals Column Liquid Chromatography

Summary

A Snyder selectivity triangle was approved for the optimization of a reversed-phases HPLC separation of haloperidol, methylparaben and propylparaben in the presence of their degradation products. The degradation products considered were: 4-(4-chlorophenyl)-4-hydroxypiperidine and 4-fluorobenzoic acid presumed to be hydrolytic products of haloperidol, and 4-hydroxybenzoic presumed to be a hydrolytic product of parabens.

Separation was achieved on an octadecyl silica colum using a mobile phase of methanol, phosphate buffer (pH 2.0) and triethylamine (50:50:0.2) with UV detection at 254 nm. The proposed method was verified for linearity, specificity, accuracy and reproducibility.

The stability-indicating capability of the assay was proved by conducting forced degradation conditions of light and temperature on a commercial drug product, for the stability investigation of the active ingredient and preservatives. It was successfully used to proceed the degradation kinetics of the drug under several storage conditions.

Key Words

Column liquid chromatography Optimization Degradation products Benzoic acids, 4-(4-Chlorophenyl)-4-hydroxypiperidine Haloperidol, methylparaben, and propylparaben 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2001

Authors and Affiliations

  • R. Driouich
    • 1
  • H. Trabelsi
    • 2
  • K. A. Bouzouita
    • 2
  1. 1.Department of Chemistry, Faculty of ScienceOkayama UniversityOkayamaJapan
  2. 2.Laboratoire National de Controle des MedicamentsTunisTunisia

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