Journal of Orthopaedic Science

, Volume 2, Issue 3, pp 171–179 | Cite as

Architectural remodeling of fresh meniscal allograft transplantation in a rabbit joint: Its long-term protective effect on articular cartilage

  • Yuichi Wada
  • Michael Amiel
  • Fred Harwood
  • David Amiel
Original Articles


This study assessed a fresh medial meniscal allograft transplantation model in the rabbit knee. Biological characterization included assessment of collagen remodeling of the allograft and the potential protection against cartilage degeneration. Allograft transplantation was performed on the left knee, and total meniscectomy on the right knee. Forty-seven rabbits were operated on and assessed at 9, 12, and 26 weeks. Fresh medial meniscal allografts showed collagen remodeling that paralleled the revascularization and cellular proliferation of the allografts. Revascularization was shown as early as 9 weeks from the periphery, extending to the inner one-third of the allograft by 26 weeks. Viability assessment of the meniscal allograft cells showed live cells at the periphery of the allograft at 9 and 12 weeks. At time 0, i.e., the time of the transplant, few viable cells were observed within the donor tissues. Biochemically, collagen remodeling, in terms of increased reducible collagen crosslinks, i.e., dihydroxylysinonorleucine, and the percentage of collagen present, was seen throughout the 26-week observation period. At 26 weeks, the meniscal allografts inhibited degenerative changes of the femoral and tibial cartialage compared to results with total meniscectomy.

Key words

transplantation allograft articular cartilage meniscus remodeling animal experiment 


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Copyright information

© The Japanese Orthopaedic Association 1997

Authors and Affiliations

  • Yuichi Wada
    • 1
  • Michael Amiel
    • 2
  • Fred Harwood
    • 2
  • David Amiel
    • 2
  1. 1.Department of Orthopaedic Surgery, School of MedicineChiba UniversityChibaJapan
  2. 2.Department of Orthopaedics, School of MedicineUniversity of California San DiegoLa JollaUSA

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