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International Journal of Clinical Oncology

, Volume 3, Issue 2, pp 108–112 | Cite as

Establishment and characterization of a novel cancer cell line (SMOV-1), derived from the ascitic fluid of a woman with ovarian cancer who had never responded to chemotherapeutic drugs

  • Tomohiro Iida
  • Kyoko Yonamine
  • Hisakiyo Takeuchi
  • Kazuhiko Hayashi
  • Takashi Meguro
  • Toshihito Shinagawa
  • Akira Amemiya
Original Article
  • 46 Downloads

Abstract

Background

A novel cell line, designated SMOV-1, was established from the ascitic fluid of a woman with ovarian cancer. This report describes the process of establishment and characterization of this cell line.

Methods

The case involved a woman who had undergone a surgical intervention, due to a serous papillary adenocarcinoma of the ovary. She received the combination chemotherapy of PAC (cisplatin +doxorubicin+cyclophosphamide) 6 times after the surgery. However, as a result of malignant pleural effusion during chemotherapy, she died 7 months after surgery. Before surgery, ascitic fluid was removed from the patient for cell culture, after obtaining her consent. The cells were successfully subcultured, and designated SMOV-1.

Results

The cell line gave a pavement-stone pattern, without contact inhibition. SMOV-1 could also be transplanted into nude mice, and the tissue showed reconstruction of the original tumor. The chromosome number of the SMOV-1 cells was 57, and the DNA index was 2.28. The population-doubling time of the SMOV-1 cells was 61.4 hours. Cultured SMOV-1 cells were still capable of producing the tumor-associated antigens CA 125, CA 19-9, and sTn (sialosyl-Tn).

Conclusion

We propose that this novel cell line is of possible use for the investigation of drug resistance in ovarian cancer.

Key words

ovarian cancer in vitro tumor marker drug resistance 

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Copyright information

© JSCO/CLJ 1988

Authors and Affiliations

  • Tomohiro Iida
    • 1
  • Kyoko Yonamine
    • 1
  • Hisakiyo Takeuchi
    • 1
  • Kazuhiko Hayashi
    • 1
  • Takashi Meguro
    • 2
  • Toshihito Shinagawa
    • 3
  • Akira Amemiya
    • 4
  1. 1.Department of Obstetrics and Gynecology St. Marianna University Yokohama City Seibu HospitalYokohamaJapan
  2. 2.Research Laboratory of St. Marianna University Yokohama City Seibu HospitalYokohamaJapan
  3. 3.Department of First Division of PathologySt. Marianna University School of MedicineKawasakiJapan
  4. 4.Department of Obstetrics and GynecologySt. Marianna University School of MedicineKawasakiJapan

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