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Inductively coupled plasma mass spectrometry for the determination of platinum accumulation in human non-small cell lung cancer cell lines

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Abstract

Background

Inductively coupled plasma mass spectrometry (ICP-MS) has been used to detect trace elements in biologic materials. In this study, we evaluated its application for the determination of cisplatin (cis-diamminedichloroplatinum) accumulation in human non-small cell lung cancer cell lines.

Methods

We used 2 squamous lung cancer cell lines (EBC-1 and LK-2), and 4 adenocarcinoma cell lines (ABC-1, RERF-LC-MS, A549, and its cisplatin-resistant subline A549/CDDP). The cells were incubated in 10 ppb cisplatin for 2 hours. After the incubation, cells were digested with nitric acid at 120°C, diluted with filtered water, and nebulized into an ICP-MS machine. A calibration curve was obtained using 5 standard platinum solutions, and cellular platinum concentrations were corrected by protein. Chemosensitivity to cisplatin was determined using the XTT assay.

Results

The cell-number detection limit for the determination of cellular platinum was 5.0×104 cells for 10 ppb cisplatin. No significant correlation was observed between cisplatin sensitivity and cellular platinum accumulation among these 6 cell lines, but an inverse correlation was observed between A549/CDDP and its parental line.

Conclusion

This study indicates that ICP-MS can be applied to the determination of platinum accumulation in human non-small cell lung cancer cell lines.

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References

  1. Morikage T, Ohmori T, Nishio K, Fujiwara Y, Takeda Y, Saijo N. Modulation of cisplatin sensitivity and accumulation by amphotericin B in cisplatin resistant human lung cancer cell lines. Cancer Res 1993;53:3302–3307.

    PubMed  CAS  Google Scholar 

  2. Ohmori T, Morikage T, Sugimoto Y, Fujiwara Y, Kasahara K, Ohta S, Takahashi T, Saijo N. The mechanism of the difference in cellular uptake of platinum derivatives in non-small cell lung cancer cell line (PC-14) and its cisplatin-resistant subline (PC-14/CDDP). Jpn J Cancer Res 1993; 84:83–92.

    PubMed  CAS  Google Scholar 

  3. Andrews PA, Howell SB. Cellular pharmacology of cisplatin: perspectives on mechanisms of acquired resistance. Cancer Cells 1990;2:35–43.

    PubMed  CAS  Google Scholar 

  4. Mckay K. New technique in the pharmacokinetic analysis of cancer drugs II. The ultratrace determination of platinum in biological samples by inductively coupled plasmamass spectrometry. Cancer Surv 1993;17:407–414.

    PubMed  CAS  Google Scholar 

  5. Lutz TM, Nivel PMV, Schmidt B. Whole blood analysis by ICP-MS. In: Holland H, Eaton AN (eds) Application of plasma source mass spectrometry. Cambridge: Royal Society of Chemistry, 1991:96–100.

    Google Scholar 

  6. Lyon TDB, Fell GS. Accuracy of multi-element analysis of human tissue obtained at autopsy using inductively coupled mass spectrometry. J Anal At Spectrom 1991; 6:559–564.

    Article  CAS  Google Scholar 

  7. Sun XF, Ting WTG, Ziesel SH, Janghorbani M. Accurate measurement of stable isotopes of lithium by inductively coupled plasma mass spectrometry. Analyst 1987;112: 1223–1228.

    Article  PubMed  CAS  Google Scholar 

  8. Scudiero DA, Shoemaker RH, Paull KD, Monks A, Tierney S, Nofziger TH, Currens MJ, Seniff D, Boyd MR. Evaluation of a soluble terazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. Cancer Res 1988;48:4827–4833.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. First Department of Medicine, Hokkaido University School of Medicine, North 15 West 7 Kita-ku, 060-8638, Sapporo, Japan

    Taro Hanada, Shigeaki Ogura & Yoshikazu Kawakami

  2. Department of Pulmonary Disease and Clinical Research Institute, Sapporo National Hospital, North 15 West 7 Kita-ku, 060-8638, Sapporo, Japan

    Hiroshi Isobe

  3. Department of Hygiene and Preventive Medicine, Hokkaido University School of Medicine, North 15 West 7 Kita-ku, 060-8638, Sapporo, Japan

    Takeshi Saitoh & Kazuo Saito

Authors
  1. Taro Hanada
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  2. Hiroshi Isobe
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  3. Takeshi Saitoh
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  4. Shigeaki Ogura
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  5. Kazuo Saito
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  6. Yoshikazu Kawakami
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Hanada, T., Isobe, H., Saitoh, T. et al. Inductively coupled plasma mass spectrometry for the determination of platinum accumulation in human non-small cell lung cancer cell lines. Int J Clin Oncol 3, 98–101 (1998). https://doi.org/10.1007/BF02492855

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  • DOI: https://doi.org/10.1007/BF02492855

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