Abstract
Several mercurials destroyed Photosystem I (PSI) Fe−S centers in thylakoids and PSI particles from spinach and fromAnacystis nidulans as revealed by EPR measurement and acid-labile sulfide determination. Of the mercurials tested, HgCl2 was the most effective, followed by phenylmercuric acetate (PMA), Mersalyl and pCMB in the order of decreasing effectiveness. Fe−S centers in thylakoids were much more labile than those in PSI particles. InA. nidulans thylakoids, Center B was more susceptible than Center A and X to PMA. P700 was less susceptible to PMA than these centers. For 50% inactivation of Fe−S centers inA. nidulans thylakoids, about 0.4 mM PMA was required for Center B, and about 1 mM was required for Center A and X. These differential susceptibilities of Fe−S centers were more pronounced with HgCl2 than with the other three mercurials.
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Abbreviations
- ALS:
-
acid-labile sulfide
- Fd:
-
ferredoxin
- FNR:
-
ferredoxin-NADP+ reductase
- HEPES:
-
N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid
- Mersaly:
-
[3-[[2-(carboxymethoxy) benzoy]amino]-2-methoxypropyl] hydroxymercury monosodium salt
- pCMB:
-
p-chloromercuribenzoate
- PMA:
-
phenylmercuric acetate
- PSI:
-
Photosystem I
- Tricine:
-
N-tris(hydroxymethyl) methylglycine
References
Golbeck, J.H., S. Lien andA. San Pietro. 1977. Isolation and characterization of a subchloroplast particle enriched in iron-sulfur protein and P700. Arch. Biochem. Biophys.178: 140–150.
— andA. San Pietro. 1976. Determination of acid-labile sulfide in subchloroplast particles containing Triton X-100. Anal. Biochem.73: 539–542.
— andJ.T. Warden. 1982. Electron spin resonance studies of the bound iron-sulfur centers in photosystem I. Photoreduction of center A occurs in the absence of center B. Biochim. Biophys. Acta681: 77–84.
Good, N.E., G.D. Winget, W. Winter, T.N. Connolly, S. Izawa andR.M.M. Singh. 1966. Hydrogen ion buffers for biological research. Biochemistry5: 467–477.
Hiyama, T., A. Murakami, S. Itoh, Y. Fujita andH. Sakurai. 1985. A low potential ironsulfur cluster bound to membranes in blue-green algae. J. Biochem.97: 89–95.
—,M. Nishimura andB. Chance. 1970. Energy and electron transfer systems ofChlamydomonas reinhardi. II. Two cyclic pathways of photosynthetic electron transfer in the pale green mutant. Plant Physiol.46: 163–168.
Honeycutt, R.C. andD.W. Krogmann. 1972. Inhibition of chloroplast reactions with phenylmercuric acetate. Plant Physiol.49: 376–380.
Inoue, K., H. Sakurai, andT. Hiyama. 1986. Photoinactivation sites of photosystem I in isolated chloroplasts. Plant Cell Physiol.27: 961–968.
Izawa, S. andN.E. Good. 1969. Effect ofp-chloromercuribenzoate (PCMB) and mercuric ion on chloroplast photophosphorylation.In: H. Metzner, ed., Progress in Photosynthesis ResearchIII: 1288–1298. Internat. Union Biol. Sci., Tübingen.
Katoh, S. andA. Takamiya 1963. Iron-protein binding in photosynthetic pyridine nucleotide reductase. Arch. Biochem. Biophys.102: 189–200.
Ke, B., E. Dolan, K. Sugahara, F.M. Hawkridge, S. Demeter andE.R. Shaw. 1977. Electro-chemical and kinetic evidence for a transient electron acceptor in the photochemical charge separation in photosystem I.In: S. Miyachiet al., ed., Photosynthetic Organelles p. 187–199. Jap. Soc. Plant Physiol., Kyoto, and Center Academic Publ. Japan Tokyo.
—,R.E. Hansen andH. Beinert. 1973. Oxidation-reduction potentials of bound ironsulfur proteins of photosystem I. Proc. Nat. Acad. Sci. USA70: 2941–2945.
Kimimura, M. andS. Katoh. 1972. Studies on electron transport associated with Photosystem I. I. Functional site of plastocyanin; Inhibitory effects of HgCl2 on electron transport and plastocyanin in chloroplasts. Biochim. Biophys. Acta283: 279–292.
Kojima, Y., T. Hiyama andH. Sakurai. 1987. Effects of mercurials on iron-sulfur centers of photosystem I ofAnacystis nidulans.In: J. Biggins, ed., Progress in Photosynthesis ResearchII, 1: 57–60. Martinus Nijhoff/Dr. W. Junk Publ., Dordrecht.
Malkin, R. 1984. Diazonium modification of photosystem I. A specific effect on iron-sulfur center B. Biochim. Biophys. Acta764: 63–69.
— andA.J. Bearden. 1971. Primary reactions of photosynthesis: Photoreduction of a bound chloroplast ferredoxin at low temperature as detected by EPR spectroscopy. Proc. Nat. Acad. Sci. USA68: 16–19.
— andJ.C. Rabinowitz. 1966. The reconstitution of clostridial ferredoxin. Biochem. Biophys. Res. Commun.23: 822–827.
McIntosh, A.R., M. Chu andJ.R. Bolton. 1975. Flash photolysis of the electron spin resonance studied of the electron acceptor species at low temperatures in photosystem I of spinach subchloroplast particles. Biochim. Biophys. Acta376: 308–314.
Ono, T. andN. Murata. 1978. Photosynthetic electron transport and phosphorylation reactions in thylakoid membranes from the blue-green algaAnacystic nidulans. Biochim. Biophys. Acta502: 477–485.
Sakurai, H. andA. San Pietro. 1985. Association of Fe−S center(s) with the large subunit(s) of photosystem I particles. J. Biochem.98: 69–76.
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Kojima, Y., Niinomi, Y., Tsuboi, S. et al. Destruction of photosystem I iron-sulfur centers of spinach andAnacystis nidulans by mercurials. Bot. Mag. Tokyo 100, 243–253 (1987). https://doi.org/10.1007/BF02492833
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DOI: https://doi.org/10.1007/BF02492833