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Chromatographia

, Volume 51, Issue 1, pp S325–S327 | Cite as

RPHPLC determination of L- and D-cystine and cysteine as cysteic acid

  • E. Varga-Visi
  • E. Terlaky-Balla
  • G. Pohn
  • L. Kamether
  • J. Csapó
Short Communications

Summary

In the quantitative analysis of cystine and cysteine, before hydrolysis the compounds are often oxidized to cysteic acid with performic acid. The applicability of this process to the quantification of the enantiomers of cystine and cysteine has been examined. An RPHPLC analytical method was developed for determination of the amount of cysteic acid enantiomers and the rate of conversion during oxidation from cystine and cysteine into cysteic acid was determined. Racemization of L-cysteine was not significant during oxidation with performic acid and the process can, therefore, be applied before hydrolysis during quantification of enantiomers of these compounds.

Key Words

Column liquid chromatography L- and D-cysteine L- and D-cystine Performic acid oxidation Cysteic acid 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2000

Authors and Affiliations

  • E. Varga-Visi
    • 1
  • E. Terlaky-Balla
    • 1
  • G. Pohn
    • 1
  • L. Kamether
    • 1
  • J. Csapó
    • 1
  1. 1.Faculty of Animal Science Kaposvar, Institute of ChemistryPannon Agricultural UniversityKaposvárHungary

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