Chromatographia

, Volume 54, Issue 5–6, pp 402–404 | Cite as

Determination of the sodium monofluoroacetate in serum by gas chromatography

  • A. C. C. O. Demarchi
  • M. L. Menezes
  • A. Mercadante
  • I. Vassillief
Short Communication Gas Chromatography

Summary

Sodium monofluoroacetate (NAFAc) has been widely used for vertebrate pest control, such as rabbits in Australia. However, NAFAc is extremely toxic to all vertebrates and its use is restricted. Although this compound is stringently restricted, the occurrence of accidental and homicidal poisoning is na ever-present possibility.

The method developed in this work shows the applicability of SPE with alumina cartridges for the extraction of NAFAc from serum samples. The method is efficient with recoveries of at least 96.8% from spiked serum. The samples were subsequently derivatized with dicyclohexyl-cabodiimide (DCC), using 2,4-dichloroaniline (DCA), to make the product volatile for GC analysis.

Key Words

Gas chromatography Sodium monofluoroacetate Rodents Alumina 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2001

Authors and Affiliations

  • A. C. C. O. Demarchi
    • 1
  • M. L. Menezes
    • 2
  • A. Mercadante
    • 3
  • I. Vassillief
    • 3
  1. 1.Laboratório de Analises e Pesquisas ClinicasUniversidade Sagrado Coração-USCSão PauloBrasil
  2. 2.Depto de Química, Av. Edmundo C. Coube S/N, BauruUniversidade Estadual Paulista-UNESPSão PauloBrasil
  3. 3.Centro de Atendimento Toxicologico, Rubião Júnior, BotucatuUniversidade Estadual Paulista-UNESPSão PauloBrasil

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