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Gas chromatographic-mass spectrometric assay for 2-methoxyethanol and 2-methoxyacetic acid in human plasma and its application to pharmacokinetic studies

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Summary

A new assay has been developed for simultaneous measurement of 2-methoxyethanol (ME) and its major toxic metabolite, 2-methoxyacetic acid (MAA) in human blood by gas chromatography-mass spectrometry. Over the concentration range 0.3 to 200 μgml−1, ME and MAA could be determined with a pooled coefficient of variation (eleven concentrations, six replicate samples) of 6.5% and 8.7%, respectively. The limits of quantification of ME and MAA were 0.19 and 0.05μg mL−1, respectively. The respective mean (SD) recoveries of ME and MAA were 101.3 (2.6)% and 98.2 (4.9)% (n=3). A study of the effects of human contact with ME liquid showed that this new method is a specific, sensitive, simple, and reliable method both for the pharmacokinetic studies and for the biological monitoring of occupational exposure to ME.

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Authors and Affiliations

  1. Institute of Occupational Safety and Health, Council of Labor Affairs, 4th Fl, No. 132 Min-Sheng E. Rd., Section 3, Taipei, Taiwan, Republic of China

    Tung-Sheng Shih, Jui-Shu Chou & Cheng-Yao Chen

  2. Department of Fire Protection, Taiwan Police College, No. 153 Shing-Lung Rd., Section 3, Taipei, Taiwan, Republic of China

    Ryh-Nan Pan

  3. Pharmaceutical Research Institute, National Defense Medical Center, P.O. Box 90048-512, Taipei, Taiwan 100, Republic of China

    Yoa-Pu Hu

Authors
  1. Tung-Sheng Shih
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  2. Ryh-Nan Pan
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  3. Jui-Shu Chou
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  4. Cheng-Yao Chen
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  5. Yoa-Pu Hu
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Shih, TS., Pan, RN., Chou, JS. et al. Gas chromatographic-mass spectrometric assay for 2-methoxyethanol and 2-methoxyacetic acid in human plasma and its application to pharmacokinetic studies. Chromatographia 54, 389–393 (2001). https://doi.org/10.1007/BF02492689

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  • DOI: https://doi.org/10.1007/BF02492689

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