Summary
A rapid and sensitive capillary electrophoretic immunoassay is described for determination of estradiol in women's serum. Addition of thermally reversible hydrogel in the buffer, serving as a replaceable packing material, improved the reproducibility of the method. Using a laser-induced fluorescence detector this method can be applied to the determination of estradiol at concentrations as low as 30.6 pg mL1. Estradiol levels in 16 normal women's serum were measured at the range 115≈370 pg mL1. The results of this method have been found to correlate well with those of chemiluminescent immunoassay.
Similar content being viewed by others
References
Sittisomwong, T.; Suneja, A.; Kudelka, A.P.; Verschraegen, C.F.; Kavanagh, J.J.Eur. J. Gynaecol. Oncol. 2000,21, 348–354.
Prestwood, K.M.; Kenny, A.M.; Unson, C.; Kulldorff, M.J. Clin. Endo. & Metab. 2000,85, 4462–4469.
Haning, R.V.; Levin, R.M.; Behrman, H.R.; Kase, N.G.; Speroff, L.J. Obst. Gynaecol. 1979,54 442–447.
Ishida, J.; Kai, M.; Ohkura, Y.J. Chromtogr. 1988,431(2), 249–257.
Stefanakis, A.; Boscos, C.; Alexopoulos, C.; Krambovitis, E.Anim. Reprod. Sci. 2000,58 (1,2), 127–135.
Nakamura, S.; Hwee, Siam, T.; Daishima, S.J. Chromatogr. A 2001,919(2), 275–282.
Geisler, J.; Berntsen, H.; Lonning, P.E.J. Steroid Biochem. Mol. Biol. 2000,72(5), 259–264.
De Boever, J.G.; Kohen, F.; Bosamns, E.Anal. Chin. Acta 1993,275, 81–87.
Spierto, F.W.; Gardner, F.; Smith, S.J.Steroids 2001,66(1), 59–62.
Sato, H.; Mochizuki, H.; Tomita, Y.; Kanamori, T.Clin. Biochem. 1996,29, 509–513.
Grossman, P.D.; Colburn, J.C.; Lauer, H.H.; Nielsen, R.G.; Riggin, R.M.; Sittampalam, G.S.; Rickard, E.C.Anal. Chem. 1989,61, 1186–1194.
Miki, S.; Kaneta, T.; Imasaka, T.J. Chromatogr. B 2001,759, 337–342.
Hanning, A.; Lindberg, P.; Westberg, J.; Roeraade, J.Anal. Chem. 2000,72, 3423–3430.
Chiem, N.; Harrison, D.J.Anal. Chem. 1997,69, 373–378.
Riechel, P.; Weiss, T.; Ulber, R.; Buchholz, H.; Scheper, T.J. Chromatogr. A 1998,817, 187–193.
Makino, K.; Suzuki, K.; Sakurai, Y.; Okano, T.; Ohshima, H.Colloids and Surf. A 1995,103, 221–226.
Huang, M.X.; Lee, M.L.J. Microcolumn Sep. 1992,4(6), 491–496.
Su, P.; Wang, Y.C.; Zhang, X.X.; Sun, L.; Chang, W.B.Anal. Chim. Acta 2000,218, 137–143.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Su, P., Zhang, XX. & Chang, WB. Application of capillary electrophoretic immunoassay to analysis of estradiol in women's serum. Chromatographia 57, 497–500 (2003). https://doi.org/10.1007/BF02492547
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02492547