Summary
An LC-MS-MS method for the simultaneous determination of DA-8159 and its active metabolite, DA-8164 in human plasma was developed. DA-8159, DA-8164 and the internal standard, sildenafil were extracted from human plasma with dichloromethane at basic pH. A reversephase HPLC separation was performed on Luna phenylhexyl column with the mixture of acetonitrile-ammonium formate (10 mM, pH 6.0) (60:40,v/v) as mobile phase. The detection of analytes was performed using an electrospray ionization tandem mass spectrometry in the multiple reaction monitoring mode. The method showed a satisfactory sensitivity (lower limits of quantification, 2.0 ng mL1), precision, accuracy, recovery and selectivity. The successful determination of DA-8159 and DA-8164 in the plasma of a volunteer who ingested a single dose of 100 mg DA-8159 confirms that the present method can be used for plasma analysis for clinical trial.
Similar content being viewed by others
References
Oh, T.Y.; Kang, K.K.; Ahn, B.O.; Yoo, M.; Kim, W.B.Arch. Pharm. Res. 2000,23, 471–476.
Choi, S.J.; Ji, H.Y.; Lee, H.-Y.; Kim, D.S.; Kim, W.B.; Lee, H.S.Biomed. Chromatogr. 2002,16, 395–399.
Shim, H.J.; Lee, E.J.; Jung, Y.H.; Kim, S.H.; Kim, S.H.; Yoo, M.; Kwon, J.W.; Kim, W.B.; Lee, M.G.J. Pharm. Biomed. Anal. 2002,30, 527–533.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kim, J., Kim, S.J., Ji, H.Y. et al. Simultaneous determination of a new phosphodiesterase-5 inhibitor DA-8159 and its active metabolite in human plasma by high performance liquid chromatography with tandem mass spectrometry. Chromatographia 57, 447–450 (2003). https://doi.org/10.1007/BF02492539
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02492539