Summary
TMS derivatives of ethoxylates and proproxylates were separated with high resolution, on standard bore and 2 mm packed columns, using both UV and FID detection. A methyl silicone column was found to dramatically decrease retention and allow the elution of heavier oligomers, compared to C18 or C8. Standard bore columns eliminated pre-column splits, and allowed full loop injections. Reproducibility for individual homologs was found to be 0.2–0.3% in retention time and 0.85 to 1.3% in area, over 10 consecutive automated injections.
The UV data was found to be equivalent to “corrected” FID data. Switching to the UV detector at 191 nm, eliminated any need for a fixed restrictor. Slow and fast program rates were shown to provide equivalent mass distribution information.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bartle, K.D.; Burke, M.P.; Clifford, A.A.; Davies, I.L.; Kithinji, J.P.; Raynor, M.W.; Shilstone, G.F.; Williams, A.Eur. Chromatogr. News 1988,2(5), 12.
Pinkston, J.D.; Bowling, D.J.; Delaney, T.E.J. Chromatogr. 1989,474, 97.
Geisler, P.R.J. Am. Oil Chem. Soc. 1989,66, 685.
Layendeker, D.; Leyendeker, D.; Hofler, F.GIT Fachz. Lab. 1989,33, 465.
Knowles, D. in “SFC Applications: 1989 Symposium on SFC”, Markides, K.E.; Lee, M.L. Eds., Brigham University Press, Provo, Utah,1989.
Gemmel, B.; Lorenschat, B.; Schmitz, F.P.Chromatographia 1989,27, 605.
Georgetti, A.; Pericles, N.; Widmer, H.M.; Anton, K.; Datwyler, P.J. Chromatogr. Sci. 1989,27, 318.
Matsumoto, K. in “SFC Applications, 1988 Workshop on Supercritical Fluid Chromatography”, compiled by: Markides, K.E.; Lee, M.L. Brigham Young Press,1988, p. 303, 305–306.
Wu, N.; Yee, R.; Lee, M.L.Chromatographia 2001,53, 197.
Gere, D.R. Hewlett-Packard Application Note1983, AN 800-3.
Miszkiewicz, W.; Hreczuch, W.; Sobczynska, A.; Szymanowski, J.Chromatographia 2000,51, 95–100.
Kamiusuki, T.; Monde, T.; Omae, K.; Morioka, K.; Konakahara, T.Chromatographia 2000,51, 390–396.
Okada, T.J. Chromatogr. 1992,609, 213.
Nozawa, A.; Ohnuma, T.J. Chromatogr. 1980,187, 261.
Meissner, Ch.; Engelhardt, H.Chromatographia,1999,49, 7–11.
Gere, D.R.Science 1983,222, 235–259.
Norris, T.A.; Rowdon, M.G.Anal. Chem. 1984,56, 1767–1769.
Berger, T.A. “Packed Column SFC”, RSC Monograph Series, Royal Society of Chemistry, Cambridge, UK,1995.
Later, D.W.; Bornhop, D.J.; Lee, E.D.; Henion, J.D.; Wiebold, R.C.LC/GC 1987,5, 804.
Guthrie, E.J.; Schwartz, H.E.J. Chromatogr. Sci. 1986,24, 236–241.
Berger, T.A.; Todd, B.S.,Chromatographia 2001,54, 771–775.
Berger, T.A.; Todd, B.S.Chromatographia 2001,54, 783–788.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Berger, T.A., Todd, B.S. Packed column supercritical fluid chromatography of oligoethers using pure carbon dioxide with flame ionization and ultraviolet detection. Chromatographia 54, 777–781 (2001). https://doi.org/10.1007/BF02492498
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02492498