, Volume 54, Issue 11–12, pp 743–747 | Cite as

Comparison of two direct methods for the determination of fatty acids in human milk

  • A. López-López
  • A. I. Castellote-Bargalló
  • M. C. López-Sabater
Originals Gas Chromatography


We have validated and compared two direct methods for determining by capillary gas chromatography fatty acid composition in human colostrum and one method that involved lipid extraction and then methylation. Direct Method 1 (DM1) consisted of transesterification of fatty acids using acetyl chloride. Direct Method 2 (DM2) used boron trifluoride-methanol as esterification reagent. The two direct methods were compared with a traditional methylation method that involves prior lipid extraction. We found that there were no differences between the direct methods and the traditional method. We then validated both direct methods; recovery rates, inter-assay and intra-assay precisions were determined with satisfactory results. Both methods gave similar results, but DM2 has certain advantages over DM1, such as speed, safety and better recovery rates.

Key Words

Gas chromatography Fatty acids Human milk Comparison of methods 


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  1. [1]
    Small, D.M.Annu. Rev Nutr. 1991,11, 413–434.CrossRefGoogle Scholar
  2. [2]
    Giovannini, M.; Riva, E.; Agostoni, C.Pediatr. Clin. North Am. 1995,42, 861–877.Google Scholar
  3. [3]
    Jensen, R.G. Handbook of milk composition. Academic Press, Inc., chapters 1 and 5,1995.Google Scholar
  4. [4]
    Gibson, R.A.; Kneebone, G.M.Am. J. Clin. Nutr. 1981,34, 252–257.Google Scholar
  5. [5]
    Boersma, E.R.; Offringa, P.J.; Muskiet, F.A.J.; Chase, W.M.; Simmons, I.J.Am. J. Clin. Nutr. 1991,53, 1197–1204.Google Scholar
  6. [6]
    Guesnet, P.; Antoine, J.M.; Rochette de Lempdes, J.B.; Galent, A.; Durand, G.Eur. J. Clin. Nutr. 1993,47, 700–710.Google Scholar
  7. [7]
    Jensen, R.G. Handbook of milk composition. Academic Press, Inc., page 508,1995.Google Scholar
  8. [8]
    Genzel-Boroviczény, O.; Wahle, J.; Koletzko, BEur. J. Pediatr. 1997,156, 142–147.CrossRefGoogle Scholar
  9. [9]
    Jansson, L.; Akesson, B.; Holmberg, L.Am. J. Clin. Nutr. 1981,34, 8–13.Google Scholar
  10. [10]
    Jackson, M.B.; Lammi-Keefe, C.J.; Jensen, R.J.; Couch, S.C.; Ferris, A.M.Am. J. Clin. Nutr. 1994,60, 353–361.Google Scholar
  11. [11]
    De la Presa-Owens, S.; López Sabater, M.C.; Rivero-Urgell, M.J.Pediatr. Gastroenterol. Nutr. 1996,22, 180–185.CrossRefGoogle Scholar
  12. [12]
    Lepage, G.; Roy, C.C.J. Lipid Res. 1986,27, 114–120.Google Scholar
  13. [13]
    Van der Steege, G.; Muskiet, F.A.J.; Martini, I.A.; Hutter, N.H.; Boersma, E.R.J. Chromatogr. 1987,415, 1–11.Google Scholar
  14. [14]
    Makrides, M.; Simmer, K.; Neumann, N.; Gibson, R.;Am. J. Clin. Nutr. 1995,61, 1231–1233.Google Scholar
  15. [15]
    Morera Pons, S.; Castellote Bargalló, A.I.; López Sabater, M.C.J. Chromatogr. A 1998,823, 475–482.CrossRefGoogle Scholar
  16. [16]
    Christie, W.W.Inform 1992,3, 1031–1034.Google Scholar
  17. [17]
    Rodriguez-Palmero, M.; López Sabater, M.C.; Castellote Bargalló, A.I.; De La Torre-Boronat, M.C.; Rivero-Urgell, M.J. Chomatogr. A 1998,793, 419–426.CrossRefGoogle Scholar
  18. [18]
    Park, P.W.; Goins, R.E.,J. Food Sci. 1994,59, 1262–1266.CrossRefGoogle Scholar
  19. [19]
    Sanjurjo Crespo, P.; Lopez de Heredia, J.; Rodriguez Alarcon, J.An. Esp. Pediatr. 1983,19, 488–493.Google Scholar
  20. [20]
    Pita, M.L.; Morales, J.; Sanchez-Pozo, A.; Martinez-Valverde, J.A.Ann. Nutr. Metab. 1985,29, 366–373.CrossRefGoogle Scholar
  21. [21]
    Domínguez Ortega, F.; Santana Reyes, C.; Reyes Suárez, D.; Quinteiro González, S.; Calvo Rosales, J.An. Esp. Pediatr. 1997,46, 455–459.Google Scholar
  22. [22]
    Rueda, R.; Ramirez, M.; Garcia-Salmeron, JL.; Maldonado, J.; Gil, A.Ann. Nutr. Metab. 1998,42, 12–22.CrossRefGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2001

Authors and Affiliations

  • A. López-López
    • 1
  • A. I. Castellote-Bargalló
    • 1
  • M. C. López-Sabater
    • 1
  1. 1.Dept. Nutrició i Bromatologia, CeRTA, Facultat de FarmàciaUniversitat de BarcelonaBarcelonaSpain

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