Summary
Insulin-like Growth Factor I is a marker of Somatotropin administration. In order to quantify this peptide in horse plasma samples, a high performance liquid chromatography/electrospray mass spectrometry method using R3 Insulin-like Growth Factor I as internal standard was developed. The extraction procedure involves an acid ethanol extraction and an immunoaffinity purification. Quantification is made possible by using deconvolution software and by measuring the insulin like growth factor-I/internal standard ratio. Comparison between high performance liquid chromatography/electrospray mass spectrometry and two immunological methods indicated agreement between methods even though more samples are needed to confirm this finding.
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Popot, M.A., Bobin, S., Bonnaire, Y. et al. High performance liquid chromatography — Ion trap mass spectrometry for the determination of insulin-like growth factor-I in house plasma. Chromatographia 54, 737–741 (2001). https://doi.org/10.1007/BF02492492
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DOI: https://doi.org/10.1007/BF02492492