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Chromatographia

, Volume 54, Issue 11–12, pp 731–736 | Cite as

Enantiomeric separation of local anaesthetic drug by HPLC on chiral stationary phases

  • C. Rustichelli
  • V. Ferioli
  • G. Gamberini
  • R. Stancanelli
Originals Column Liquid Chromatography

Summary

High-performance liquid chromatographic methods on chiral stationary phases have been developed for the stereoselective separations of the enantiomers of articaine hydrochloride and its metabolite articainic acid.

Complete separations of articaine enantiomers have been achieved on a Chiralcel OD analytical column using mobile phases containing n-hexane and 2-propanol, while articainic acid enantiomers were resolved with n-hexane and ethanol.

Good results were also obtained on a Chirobiotic V analytical column with 5% acetonitrile in triethylammonium acetate solution (7 mM; pH 6.5); the column showed good chemioselectivity and stereoselectivity, allowing simultaneous separation of both the enantiomeric pairs of articaine and articainic acid.

Key Words

Column liquid chromatography Cellulose-based chiral stationary phase Vancomycin bonded-phase column Articaine and articainic acid 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2001

Authors and Affiliations

  • C. Rustichelli
    • 1
  • V. Ferioli
    • 1
  • G. Gamberini
    • 1
  • R. Stancanelli
    • 2
  1. 1.Dipartimento di Scienze FarmaceuticheUniversità di Modena e Reggio EmiliaModena (MO)Italy
  2. 2.Dipartimento Farmaco-ChimicoUniversità di MessinaMessina (ME)Italy

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