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Enantiomeric separation of local anaesthetic drug by HPLC on chiral stationary phases

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Summary

High-performance liquid chromatographic methods on chiral stationary phases have been developed for the stereoselective separations of the enantiomers of articaine hydrochloride and its metabolite articainic acid.

Complete separations of articaine enantiomers have been achieved on a Chiralcel OD analytical column using mobile phases containing n-hexane and 2-propanol, while articainic acid enantiomers were resolved with n-hexane and ethanol.

Good results were also obtained on a Chirobiotic V analytical column with 5% acetonitrile in triethylammonium acetate solution (7 mM; pH 6.5); the column showed good chemioselectivity and stereoselectivity, allowing simultaneous separation of both the enantiomeric pairs of articaine and articainic acid.

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Authors and Affiliations

  1. Dipartimento di Scienze Farmaceutiche, Università di Modena e Reggio Emilia, via Campi, 183, 41100, Modena (MO), Italy

    C. Rustichelli, V. Ferioli & G. Gamberini

  2. Dipartimento Farmaco-Chimico, Università di Messina, Vill. SS. Annunziata, 98168, Messina (ME), Italy

    R. Stancanelli

Authors
  1. C. Rustichelli
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  2. V. Ferioli
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  3. G. Gamberini
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  4. R. Stancanelli
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Rustichelli, C., Ferioli, V., Gamberini, G. et al. Enantiomeric separation of local anaesthetic drug by HPLC on chiral stationary phases. Chromatographia 54, 731–736 (2001). https://doi.org/10.1007/BF02492491

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  • DOI: https://doi.org/10.1007/BF02492491

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