Summary
A 94.2% pure fraction of methyl-GLA has been isolated in 84.6% yield from extracts ofAnchusa azurea seed oil (Boraginaceae) by means of a four-step process:
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(i)
simultaneous extraction and saponification of the seeds;
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(ii)
concentration of polyunsaturated fatty acids (PUFA) by the urea concentration method;
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(iii)
urea-concentrate methylation; and
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(iv)
high performance liquid chromatographic (HPLC) fractionation of the PUFA ester concentrate.
When same procedure was then tested for the fractionation ofEchium fastuosum (Boraginaceae) seed oil extracts methyl-GLA fractions of almost 100% purity were obtained, although yields were low, because of the presence of significant amounts of α-linolenic acid (ALA) in the oil.
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Campra-Madrid, P., Guil-Guerrero, J.L. High-performance liquid chromatographic purification of γ-linolenic acid (GLA) from the seed oil of two Boraginaceae species. Chromatographia 56, 673–677 (2002). https://doi.org/10.1007/BF02492467
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DOI: https://doi.org/10.1007/BF02492467