Simplification of a chromatographic test methodology for evaluation of base deactivated supports
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Today reversed-phase liquid chromatography (RP-LC) is the most employed chromatographic technique for analysis of basic compounds. Unfortunately, the strong ionic interactions of basic compounds with residual silanols result in asymmetric peaks and non-reproducible retention. This problem and the continous increase in the use of RP-LC techniques have furthered the need for a new generation of “base deactivated” stationary phases.
Selecting the appropriate stationary phase for a specific separation is an important parameter in the development of LC methods. To characterize and evaluate the relative chromatographic performance of stationary phases, a series of tests are proposed in the literature.
In this work, a chromatographic test previously developed is discussed with the aim of simplifying its methodology. The number of test compounds was reduced (seven instead of fourteen), as well as that of the mobile phases (one or two instead of three) and columns per support (three instead of five).
This simplification allows a faster and easier method to evaluate new chromatographic supports.
Key WordsColumn liquid chromatography Principal component analysis Chromatographic test method Basic compounds
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