Advertisement

Chromatographia

, Volume 55, Issue 1–2, pp 63–68 | Cite as

Determination of isoquinoline alkaloids inThalictrum herbal drugs by non-aqueous capillary electrophoresis

  • Xiaodan Su
  • Tao Bo
  • Runkai Li
  • Ke An Li
  • Huwei Liu
Originals Electrophoresis

Summary

A rapid non-aqueous capillary electrophoresis method has been developed for the separation and determination, within 14 min, of eight isoquinoline alkaloids (berberine, palmatine, jatrorrhizine, (+)-tetrandrine, berbamine, thalifaricine, northalfine, and thalistine) in seventeen samples of the herbal drug thalictrum. A methanolic solution of sodium acetate (75mm) and acetic acid (1m) was found to be the optimum running buffer for the separation. Thalictrum Atriplex Finet et Gagep (T.AFG) was selected for further study, including investigation of recovery and precision, because this preparation contained all the isoquinoline alkaloids tested. Calibration curves were highly linear over a 20-fold concentration range and detection limits for all eight alkaloids were in the range 0.42–3.04 μg mL−1.

Key Words

Capillary electrophoresis Non-aqueous buffer Thalictrum Isoquinoline alkaloids 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    Gao, G.Y.; PhD Dissertation, Study of the Chemical Components of T. Atriplex Finet et Gagep, Institute of Medicinal Plant Development, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China,1995.Google Scholar
  2. [2]
    Zhou, Y.; Guo, Y.Z.; Meng, X.S.Chinese Herbal Medicine 1990,21(9), 13.Google Scholar
  3. [3]
    Wang, X.X.; Guo, Y.Z.Shenyang Yaoxue Xuebao (Chinese)1992,9(1), 22.Google Scholar
  4. [4]
    Zhou, Y.; Guo, Y.Z.; Meng, X.S.Shenyang Yaoxue Xuebao (Chinese)1991,8(4), 291.Google Scholar
  5. [5]
    Wang, Z.T.; Xiao, P.G.Chinese Herbal Medicine 1988,19(4), 161.Google Scholar
  6. [6]
    Zhu, M.Yaowu Fenxi Zazhi (Chinese)1990,10(2), 72.Google Scholar
  7. [7]
    Wei, W.; Wang, Y.M.; Luo, G.A.Acta Pharm. Sinica 1997,32, 476.Google Scholar
  8. [8]
    Sheu, S.J.Chemistry (Taiwan)1996,54, 55.Google Scholar
  9. [9]
    Li, Y.; Li, Y.; Liu, H.Mod. Instrum. (Chinese)2000,5, 1.Google Scholar
  10. [10]
    Li, Y.; Zou, C.; Liu, H.Chromatographia 1999,50, 358.CrossRefGoogle Scholar
  11. [11]
    Li, Y.; Ji, X.; Liu, H.Chromatographia 2000,51, 357.CrossRefGoogle Scholar
  12. [12]
    Li, Y.; Long, H.; Liu, H.J. Chin. Pharm. Sci. 2000,9, 80.Google Scholar
  13. [13]
    Ji, X.; Li, Y.; Liu, H.; Yan, H.; Yan, Y.; Li, J.J. Pharm. Acta Helv. 2000,74, 387.CrossRefGoogle Scholar
  14. [14]
    Su, X.; Wang, X.; Li, Y.; Li, J.; Yan, Y.; Chen, Y.; Liu, H.J. Microcol. Sep. 2001,13, 221.CrossRefGoogle Scholar
  15. [15]
    Bjornsdottir, I.; Hansen, S.H.J. Pharm. Biomed. Anal. 1995,13, 687.CrossRefGoogle Scholar
  16. [16]
    Trenerry, V.G.; Well, R.J.; Robertson, J.J. Chromatogr. A 1995,718, 217.CrossRefGoogle Scholar
  17. [17]
    Bjornsdottir, I.; Hansen, S.H.J. Pharm. Biomed. Anal. 1995,13, 1473.CrossRefGoogle Scholar
  18. [18]
    Liu, Y.M., Sheu, S.J.J. Chromatogr. 1993,639, 323.CrossRefGoogle Scholar
  19. [19]
    Pietta, P.G.; Mauri, P.L.; Gardana, C.; Colombo, M.L.; Tome, F.Phytochem. Anal. 1995,6, 196.CrossRefGoogle Scholar
  20. [20]
    Liu, Y.M.; Sheu, S.J.J. Chromatogr. 1992,600, 370.CrossRefGoogle Scholar
  21. [21]
    Riekkola, M.L.; Jussila, M.; Porras, S.P.; Valko, L.E.J. Chromatogr. A 2000,892; 155.CrossRefGoogle Scholar
  22. [22]
    Siren, H.; Hiissa, T.; Min, Y.Analyst 2000,125, 1561.CrossRefGoogle Scholar
  23. [23]
    Kreft, S.; Zel, J.; Pukl, M.; Umek, A.; Strkelj, B.Phytochem. Anal. 2000,11, 37CrossRefGoogle Scholar
  24. [24]
    Li, S.G.; Chai, Y.F.; Zhang, G.Q.; Wu, Y.T.; Liang, D.S.; Xu, Z.M.Biomed. Chromatogr. 1999, 13, 439.CrossRefGoogle Scholar
  25. [25]
    Tjornelund, J.; Hansen, S.H.J. Biochem. Biophys. Methods 1999,38, 139.CrossRefGoogle Scholar
  26. [26]
    Leung, G.; Tang, H.; Tso, T.; Wan, T.J. Chromatogr. A 1996,738, 141.CrossRefGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2002

Authors and Affiliations

  • Xiaodan Su
    • 1
  • Tao Bo
    • 1
  • Runkai Li
    • 1
  • Ke An Li
    • 1
  • Huwei Liu
    • 1
  1. 1.Department of ChemistryPeking UniversityBeijingP.R. China

Personalised recommendations