Development of rigid biporous polymeric adsorbent for protein chromatography

Summary

A novel biporous poly (glycidyl methacrylate-triallyl isocyanurate-divinylbenzene) resin (denoted as Resin C) was prepared byin-situ co-polymerization with both superfine granules of sodium sulfate and the solvents toluene and n-heptane as porogenic agents. This material is based on a novel porogenic mode of a combination of both solid granules and solvents. The properties of Resin C were characterized, and then compared with both Resin A (where only solvents as porogen) and Resin B (where only solid granules are used as porogen). The biporous resin showed good mechanical performance, high dynamic adsorption capacity and column efficiency at high flow-rates. These factors indicate the biporous resin as a promising adsorbent for high-speed protein separation by chromatography.

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Shi, Y., Dong, X.Y. & Sun, Y. Development of rigid biporous polymeric adsorbent for protein chromatography. Chromatographia 55, 405–410 (2002). https://doi.org/10.1007/BF02492268

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Key words

  • Column liquid chromatography
  • Biporous matrix
  • In situ co-polymerization
  • Proteins