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Chromatographia

, Volume 55, Issue 7–8, pp 405–410 | Cite as

Development of rigid biporous polymeric adsorbent for protein chromatography

  • Y. Shi
  • X. -Y. Dong
  • Y. Sun
Originals Column Liquid Chromatography

Summary

A novel biporous poly (glycidyl methacrylate-triallyl isocyanurate-divinylbenzene) resin (denoted as Resin C) was prepared byin-situ co-polymerization with both superfine granules of sodium sulfate and the solvents toluene and n-heptane as porogenic agents. This material is based on a novel porogenic mode of a combination of both solid granules and solvents. The properties of Resin C were characterized, and then compared with both Resin A (where only solvents as porogen) and Resin B (where only solid granules are used as porogen). The biporous resin showed good mechanical performance, high dynamic adsorption capacity and column efficiency at high flow-rates. These factors indicate the biporous resin as a promising adsorbent for high-speed protein separation by chromatography.

Key words

Column liquid chromatography Biporous matrix In situ co-polymerization Proteins 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2002

Authors and Affiliations

  • Y. Shi
    • 1
  • X. -Y. Dong
    • 1
  • Y. Sun
    • 1
  1. 1.Department of Biochemical Engineering, School of Chemical Engineering and TechnologyTianjin UniversityTianjinP.R. China

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