Separation of chiral pharmaceuticals using ultrahigh pressure liquid chromatography
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Ultrahigh pressure liquid chromatography was demonstrated for fast and efficient chiral separations. Capillary columns approximately 13–24 cm in length packed with nonporous 1.0μm C6-modified silical particles were used. β-Cyclodextrin (β-CD) and 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) were added to the mobile phase as modifiers to produce transient diastereomeric complexes with the analytes. Pressures up to ≈42,000 psi were applied, and efficiencies in excess of 200,000 plates m−1 were obtained for separations that were accomplished in less than 2 minutes.
Key wordsColumn liquid chromatography Ultrahigh pressure chromatography Chiral drugs β-Cyclodextrin 2-Hydroxypropyl-β-cyclodextrin
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