, Volume 55, Issue 7–8, pp 399–403 | Cite as

Separation of chiral pharmaceuticals using ultrahigh pressure liquid chromatography

  • Y. Xiang
  • N. Wu
  • J. A. Lippert
  • M. L. Lee
Originals Column Liquid Chromatography


Ultrahigh pressure liquid chromatography was demonstrated for fast and efficient chiral separations. Capillary columns approximately 13–24 cm in length packed with nonporous 1.0μm C6-modified silical particles were used. β-Cyclodextrin (β-CD) and 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) were added to the mobile phase as modifiers to produce transient diastereomeric complexes with the analytes. Pressures up to ≈42,000 psi were applied, and efficiencies in excess of 200,000 plates m−1 were obtained for separations that were accomplished in less than 2 minutes.

Key words

Column liquid chromatography Ultrahigh pressure chromatography Chiral drugs β-Cyclodextrin 2-Hydroxypropyl-β-cyclodextrin 


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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2002

Authors and Affiliations

  • Y. Xiang
    • 1
  • N. Wu
    • 2
  • J. A. Lippert
    • 3
  • M. L. Lee
    • 1
  1. 1.Department of Chemistry and BiochemistryBrigham Young UniversityProvoUSA
  2. 2.Merck Research LaboratoriesAnalytical Research DepartmentRahwayUSA
  3. 3.Department of ChemistryWeber State UniversityOgdenUSA

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