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Chromatographia

, Volume 54, Issue 3–4, pp 163–167 | Cite as

Determination of paracetamol (acetaminophen) by HPLC with post-column enzymatic derivatization and fluorescence detection

  • J. Meyer
  • U. Karst
Originals

Summary

A novel method is described for the determination of paracetamol (acetaminophen;N-acetyl-4-aminophenol) in urine. After reversed-phase HPLC separation, paracetamol is oxidized by H2O2 with horseradish peroxidase catalysis. Detection is performed fluorimetrically at an excitation wavelength of 329 nm and an emission wavelength of 435 nm. Urine samples were spiked with paracetamol, diluted, and injected directly without further pretreatment. Under these conditions, the limit of detection was 2×10−8 molL−1, and the limit of quantification was 7×10−8 molL−1. The method was validated by two different approaches based on HPLC with UV-Vis detection.

Key Words

Column liquid chromatography Post-column derivatization Fluorescence detection Paracetamol Urine 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2001

Authors and Affiliations

  • J. Meyer
    • 1
  • U. Karst
    • 1
  1. 1.Anorganisch-Chemisches InstitutWestfälische Wilhelms-Universität MünsterMünsterGermany

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