Simultaneous determination of benzene and total aromatic fraction of gasoline by HPLC-DAD
A new method is described for the simultaneous determination of benzene and the total aromatic fraction in gasoline by HPLC coupled with a diode array detector (DAD). The detection wavelength of 205 nm was selected on the basis of the UV spectra of benzene, its homologues and interfering compounds, obtained by diode array detector. The use of this wavelength plays an important role in minimizing positive interference from trace compounds, which have a strong absorbance at 254 nm, the typical wavelength at which aromatics are detected.
To develop the method a standard solution of seven aromatics compounds was used (benzene, toluene, ortho-, meta- and para-xylenes, ethyl-benzene and 1,3,5-trimethyl-benzene), each at 5% v/v in hexane to simulate the behavior of a gasoline with 35% v/v of total aromatics.
The sensitivity of this method to benzene is in the range of 2×10−3% v/v. This method was also applied to a gasoline with a known percentage of benzene and total aromatics and proved quite accurate.
Key WordsColumn liquid chromatography Gasoline Benzene Total aromatic fraction
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