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Optimized separation of pharmacologically active xanthones fromSecuridaca inappendiculata by capillary electrophoresis

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  • Electrochromatography/Electrophoresis
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Summary

A capillary electrophoresis (CE) method with diode-array detection (DAD) has been developed for the separation of the therapeutically important xanthones fromSecuridaca inappendiculata for the first time, based on the systematic optimization of such parameters as pH, concentration of running buffers, addition of sulfated β-CD, applied voltage and column temperature. Baseline separation was achieved for the nine xanthones in less than 15 min using a background electrolyte consisting of 200 mM borate (pH 9.5) and 10 mM sulfated β-CD. Apparent analyte—selector binding constants between sulfated β-CD and xanthones were calculated to elucidate the migration order and the separation mechanism.

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Authors and Affiliations

  1. Chemistry Department, Peking University, 10087, Beijing, P.R. China

    Tao Bo, Fei Gao, Huwei Liu & Ke An Li

  2. Institute of Medicinal Plant Development, Peking Union Medical College and Chinese Academy of Medical Sciences, 100094, Beijing, P.R. China

    Xuedong Yang & Lizhen Xiu

Authors
  1. Tao Bo
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  2. Xuedong Yang
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  3. Fei Gao
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  4. Huwei Liu
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  5. Ke An Li
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  6. Lizhen Xiu
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Bo, T., Yang, X., Gao, F. et al. Optimized separation of pharmacologically active xanthones fromSecuridaca inappendiculata by capillary electrophoresis. Chromatographia 55, 217–223 (2002). https://doi.org/10.1007/BF02492145

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  • DOI: https://doi.org/10.1007/BF02492145

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