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Novel approach for the purification and analysis of methotrexate-branched polymeric polypeptide conjugates by HPLC

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Summary

Methotrexate (MTX) has been coupled to various structurally related, polycationic synthetic branched polypeptides containing a poly[L-Lys] backbone. We describe here a novel, rapid and simple RP-HPLC approach and its application for a) the monitoring of MTX-macromolecule conjugate synthesis, b) analysis of the composition of conjugates samples, c) semi-preparative purification of target compounds from reagents and side products, and d) stability studies of MTX-branched polypeptide conjugate samples.

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References

  1. Ghose, T.; Blair, A.H.; Kralovec, J.; Mammen, M.; Uadia, P.O. InAntibodymediated delivery systems: Rodwell, J.D., Ed. Marcel Dekker, New York,1988.

    Google Scholar 

  2. Furst, D.E.; Kremer, J.M.Arthritis Rheum. 1988,31, 305–314.

    CAS  Google Scholar 

  3. Vergne, P.; Liagre, B.; Bertinm, P.; Cook-Moreau, J.; Treves, R.; Beneytout, J.L.; Rigaud, M.J. Rheumatol. 1998,25, 433–440.

    CAS  Google Scholar 

  4. Mukhopadhyay, A.; Chaudhuri, G.; Arora, S.K.; Sehgal, S.; Basu, S.K.Science 1989,244, 705–707.

    CAS  Google Scholar 

  5. Endo, N.; Takeda, Y.; Umemoto, N.; Kishida, K.; Watanabe, K.; Saito, M.; Kato, Y.; Hara, T.Cancer Res. 1988,48, 3330–3335.

    CAS  Google Scholar 

  6. Kulkarni, P.N.; Blair, A.H.; Ghose, T.I.Cancer Res. 1981,41, 2700–2706.

    CAS  Google Scholar 

  7. Halbert, G.W.; Florence, A.T.J. Pharm. Pharmacol. 1989,41, 222–226.

    CAS  Google Scholar 

  8. Garnett, M.C.; Baldwin, R.W.Cancer Res. 1986,46, 2407–2412.

    CAS  Google Scholar 

  9. Endo, N.; Kato, Y.; Takeda, Y.; Saito, M.; Umemoto, N.; Kishida, K.; Hara, T.Cancer Res. 1987,47, 1076–1080.

    CAS  Google Scholar 

  10. Stehle, G.; Sinn, H.; Wunder, A.; Schrenk, H.H.; Schutt, S.; Maier Borst, W.; Heene, D.L.Anticancer Drugs 1997,8, 677–685.

    CAS  Google Scholar 

  11. Kosasih, A.; Bowman, B.J.; Wigent, R.J.; Ofner, C.M.Int. J. Pharm. 2000,204, 81–89.

    Article  CAS  Google Scholar 

  12. Chakraborty, P.; Bhaduri, A.N.; Das, P.K.Biochem. Biophys. Res. Commun. 1990,166, 404–410.

    Article  CAS  Google Scholar 

  13. Ryser, H.J.P.; Shen, W.C.Proc. Nat. Acad. Sci. USA 1978,75, 3867–3870.

    Article  CAS  Google Scholar 

  14. McGuire, J.J.; Russell, C.A.Leukemia 1990,4, 48–52.

    CAS  Google Scholar 

  15. Hudecz, F.; Clegg, J.A.; Kajtár, J.; Embleton, M.J.; Pimm, M.V.; Szekerke, M.; Baldwin, R.W.Bioconjugate Chem. 1993,4, 25–33.

    Article  CAS  Google Scholar 

  16. Sanzgiri, Y.; Blaton, C.D.; Gallo, J.M.Pharmaceutical Res. 1990,7, 418–421.

    Article  CAS  Google Scholar 

  17. Manabe, Y.; Tsubota, T.; Haruta, Y.; Kataoka, K.; Okazaki, M.; Haisa, S.; Nakamura, K.; Kimura, I.J. Lab. Clin. Med. 1984,104, 445–454.

    CAS  Google Scholar 

  18. Przybylski, M.; Fell, E.; Ringsdorf, H.; Zaharko, D.S.Makromol. Chem 1978,179, 1719–1733.

    Article  CAS  Google Scholar 

  19. Tsou, H.R.; Wallace, R.; Citarella, R.V.; Upeslacis, J. InPolymeric Drugs and Drug Delivery Systems: Dunn, R.L.; Ottenbrite R.O., Eds, ACS Symposium Series 469, ACS, Washington DC,1991.

    Google Scholar 

  20. Kralovec, J.; Singh, M.; Mammen, M.; Blair, A.H.; Ghose, T.Cancer Immunol. Immunother. 1989,29, 293–302.

    Article  CAS  Google Scholar 

  21. Bures, L.; Lichy, A.; Bostik, J.; Spundova, M.Neoplasma 1990,37, 225–231.

    CAS  Google Scholar 

  22. McCrudden, E.A.; Tett, S.E.,J. Chromatogr. B Biomed. Sci. Appl. 1999,721, 87–92.

    Article  CAS  Google Scholar 

  23. Elias, D.J.; Hirschowitz, L.; Kline, L.E.; Koener, J.F.; Dillman, R.O.; Walker, L.E.; Robb, J.A.; Timms, R.M.Cancer Research 1990,50, 4154–4159.

    CAS  Google Scholar 

  24. Hudecz, F.; Garnett, M.C.; Khan, T.; Baldwin, R.W.Biomed. Chromatography 1992,6, 128–132.

    Article  CAS  Google Scholar 

  25. Mező G.; Kajtár, J.; Hudecz, F.; Szekerke, M.Biopolymers 1993,33, 873–883.

    Article  Google Scholar 

  26. Hudecz, F.; Pimm, M.V.; Rajnavölgyi, É.; Mező, G.; Fabra, A.; Gaál, D.; Kovács, A.L.; Horváth, A.; Szekerke, M.Bioconjugate Chem. 1999,10, 781–790.

    Article  CAS  Google Scholar 

  27. Mező, G.; Kajtár, J.; Nagy, I.; Szekerke, M.; Hudecz, F.Biopolymers 1997,42, 719–730.

    Article  Google Scholar 

  28. Hudecz, F.; Kovács, P.; Kutassi-Kovács, S.; Kajtár, J.Colloid and Polymer Sci. 1984,262, 208–212.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Research Group of Peptide Chemistry, Eötvös L. University, Hungarian Academy of Sciences, P.O. B. 32, 1518, Budapest 112, Hungary

    G. Kóczán & F. Hudecz

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  1. G. Kóczán
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  2. F. Hudecz
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Kóczán, G., Hudecz, F. Novel approach for the purification and analysis of methotrexate-branched polymeric polypeptide conjugates by HPLC. Chromatographia 55, 163–169 (2002). https://doi.org/10.1007/BF02492137

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  • DOI: https://doi.org/10.1007/BF02492137

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