Summary
A novel variant of continuous relative counter-current extraction, called medium-pressure solid-liquid extraction (MPSLE) is introduced as a new preparative separation method for laboratory purposes. This method is a special case of exhaustive extraction.
On using MPSLE, the extraction column, i.e. a medium pressure liquid chromatographic (MPLC) column is filled with the fine-powdered solid phase to be extracted, and extraction solvent(s) is/are forced through the stationary bed by means of a pump. This method constitutes the relative counter-current extraction, and results in exhaustive and rapid extraction. By MPSLE, efficiency is achieved not just because the extraction solvent(s) is/are forced through the staying material to be extracted, but also due to the overpressure in the extraction column and the suitable changes of the dissolution and diffusion periods of the process.
The method can be used for the rapid extraction of various substance classes occurring as complex solid matrices. Experiments carried out with MPLC columns show that the exhaustive extraction of finely powdered plant material of 100–3000 g can be extracted and concentrated with an automated equipment within a few hours. Further advantages of the environment-friendly process performed in closed system are full automation and operation with low quantity of extractant.
Similar content being viewed by others
References
Hostettmann, K.; Marston, K.; Hostettmann, M.Preparative Chromatography Techniques, Second, Completely Revised and Enlarged Edition, Springer, Berlin,1997.
List, W.; Schmidt, P.C.Technologie pflanzlicher Arzneizubereitungen, Wissenschaftliche Verlagsgesellschaft mbH, Stuttgart,1989, pp. 85–98.
Nyiredy, Sz. In:Planar Chromatography— A Retrospective View for the Third Millennium: Nyiredy, Sz., Ed., Springer, Budapest,2001, pp. 177–199.
Nyiredy, Sz.Trends Anal. Chem. 2001,20, 91–101.
Zogg, G.; Nyiredy, Sz.; Sticher, O.J. Liq. Chromatogr. 1989,12, 2031–2048.
Zogg, G.; Nyiredy, Sz.; Sticher, O.J. Liq. Chromatogr. 1989,12, 2049–2065.
Nyiredy, Sz.; Botz, L.; Sticher, O.J. Chromatogr. 1990,523, 43–52.
Nyiredy, Sz.Chromatographia 2000,51, S288-S296.
Snyder, L.J. Chromatogr. Sci. 1978,16, 223–234.
Nyiredy, Sz.J. Chromatogr. Sci. 2002,40, 553–563.
Nyiredy, Sz.J. Planar Chromatogr. 2001,14, 393–395.
Zogg, G.C.Optimization and Transfer of the Mobile Phase between Various Analytical and Preparative Chromatographic Methods, Diss ETH No. 8780, Zurich,1989.
Dallenbach-Toelke, K.Anwendung des «PRISMA»-Modells auf verschiedene chromatographische Methoden bei polaren Inhaltsstoffen von Pharmakopöe-Drogen, Diss ETH No. 8178, Zurich,1986.
Nyiredy, Sz.; Botz L.; Sticher, O.Swiss Patent Nr. CH 673405.
Nyiredy, Sz.J. AOAC Int. 2001,84, 1219–1231.
Vovk, I.; Simonovska, B.; Andrensek, A.; Yrjönen, T.; Vuorela, P.; Vuorela, H.Proceedings Book of the International Symposium Planar Chromatography Today 2002, National Institute of Chemistry, Ljubljana,2002, pp. 301–314.
Vovk, I.; Simonovska, B.; Andrensek, A.; Yrjönen, T.; Vuorela, P.; Vuorela, H.J. Planar Chromatogr. 2003,16, 46–51.
in preparation.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nyiredy, S., Botz, L. Medium-pressure solid-liquid extraction: A new preparative method based on the principle of counter-current. Chromatographia 57 (Suppl 1), S291–S295 (2003). https://doi.org/10.1007/BF02492118
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02492118