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Chromatographia

, Volume 57, Supplement 1, pp S207–S212 | Cite as

Chiral gas chromatographic analysis of amino acids in fortified wines

  • R. Pätzold
  • A. Nieto-Rodriguez
  • H. Brückner
Originals Gas Chromatography

Summary

Free amino acids in fortified (dessert) wines (n=41) from Spain (sherry, malaga, moscatel), Portugal (port, madeira), Italy (marsala), Greece (mavrodaphne), and liquor wines from Hungary (tokay) and Israel were analyzed for D amino acids. Amino acids were isolated from wines using Dowex 50W X8 cation exchanger and converted into volatileN(O)-pentafluoropropionyl amino acid I-propyl esters. Enantiomers were separated by gas chromatography on Chirasil-L-Val capillary columns and relative quantities ofd amino acids wee determined by selected-ion monitoring mass spectrometry or flame-ionization detection. Major quantities ofd-Ala (4.0–58.1%),d-Asp (8.9–51.3%),d-Glu (4.9–28.4%), andd-Ser (0.4–14.7%) were detected in all wines and minor amounts (<1%) ofd-Lys,d-Phe,d-Tyr, andd-Val in some wines. Notably, quantities ofd-Ala exceeded 50% of total amino acids in some fortified wines.

Key Words

Gas chromatography-mass spectrometry Enantiomeric separation Amino acids Fortified wines Maillard reaction 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 2003

Authors and Affiliations

  • R. Pätzold
    • 1
  • A. Nieto-Rodriguez
    • 1
  • H. Brückner
    • 1
  1. 1.Intersisciplinary Research Center, Institute of Nutritional Science, Department of Food SciencesJustus-Liebig-University of GiessenGiessenGermany

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